摘要
目的以大鼠组织匀浆及ＲＢＣ为材料研究３种穿心草口山酮（ｘａｎｔｈｏｎｅ，Ｘａｎ）：１，８－ｄｉｈｙｄｒｏｘｙ３，５－ｄｉｍｅｔｈｏｘｙｘ－ａｎｔｈｏｎｅ（Ｘａｎ－Ⅰ）；１－ｈｙｄｒｏｘｙ，５－ｄｉｍｅｔｈｏｘｙｘａｎｔｈｏｎｅ（Ｘａｎ－Ⅱ）；１－ｈｙｄｒｏｘｙ，３，７，８－ｔｒｉｔｈｏｘｙｘａｎｔｈｏｎｅ（Ｘａｎ－Ⅲ）抗脂质过氧化作用。方法以ＭＤＡ为指标，观察Ｘａｎ对组织匀浆体外脂质过氧化及ＲＢＣ自氧化溶血体系脂质过氧化产物丙二醛（ＭＤＡ）生成量的影响；并观察Ｘａｎ对邻苯三酚自氧化产生的Ｏ·２及Ｃｕ２＋－ＶｉｔＣ自由基产生系统产生的·ＯＨ的清除作用。结果①０．０５μｍｏｌ·Ｌ－１的３种Ｘａｎ均可抑制正常大鼠脑、肝、心匀浆体外过氧化脂质生成，并能对抗半胱氨酸和硫酸亚铁所致过氧化脂质生成增加。②３种Ｘａｎ均能抑制ＲＢＣ自氧化，显著减少ＲＢＣ自氧化过程中ＭＤＡ的含量。③３种Ｘａｎ均能清除超氧阴离子自由基（Ｏ·２）和羟自由基（·ＯＨ），清除·ＯＨ的作用更强。④３种Ｘａｎ清除氧自由基的活性强弱顺序为Ｘａｎ－Ⅰ＞Ｘａｎ－Ⅲ＞Ｘａｎ－Ⅱ。结论穿心草口山酮具有一定的抗氧化作用，能直接清除活性氧自由基。
AIM To study antioxidation of canscora luciadissima xanthone(Xan):1,8dihydroxy,3,5dimethoxyxanthone(XanⅠ); and 1hydroxy,5dimethoxyxanthone(XanⅡ);1hydroxy,3,7,8trithoxyxanthone(XanⅢ) on homogenate and RBC autoxidation. METHODS Samples of tissues homogenates and RBC suspension were pretreated with Xans. The generation of malondialdehyde(MDA) was examined. The generation of O·2 produced by pyrogallol autoxidation and the ·OH produced by Cu2+Vit C were examined by being pretreated with Xans. RESULTS ① The production of lipid peroxides in normal brain, liver and heart homogenate of rat and elevation of production of lipid peroxides induced by cysteine and FeSO4 in homogenate were inhibited by addtion of Xans. ② Xan inhibited that RBC hemolysis and the generation of MDA was decreased. ③ The O·2 produced by autoxidation of pyrogallol and the ·OH produced by Cu2+Vit C system could be scavenged by Xans. ④ The potency of scavenging oxigen free radal is that XanⅠ>XanⅢ>XanⅡ among the Xans. CONCLUSION Canscora lucidissima xanthones have the effect of antilipid peroxidation and may scavenge both O·2 and ·OH.
出处
《中国药理学通报》
CAS
CSCD
北大核心
1998年第2期130-132,共3页
Chinese Pharmacological Bulletin
关键词
穿心草
SHAN酮
抗氧化作用
植物药
canscora lucidissima, xanthone(Xan)
malondialdehyde(MDA)
active oxygen species
lipid peroxidation
