摘要
目的建立测定白芍药材中芍药苷和五没食子酰葡萄糖含量的双波长RP—HPLC分析方法。方法采用HypersilODS（4．6×250mm，5μm）色谱柱，以乙腈和0．05％磷酸为流动相，梯度洗脱，流速1．0mL/min，检测波长为230nm和275nm，柱温25℃。结果芍药苷在0．208～2．08μg呈良好的线性关系，r=0．9999，平均回收率96．3％；五没食子酰葡萄糖在0．04～0．4μg呈良好的线性关系，r=0．9998，平均回收率98．9％。结论该方法简便、快速、准确、灵敏度高，可用于白芍的质量控制。
Objective To establish a dual wavelength method of RP-HPLC for the quantitative determination of paeoniflofin and pentagalloylglucose in Paeonia lactiflora Pall. Methods The dual wavelength method of RP-HPLC was performed on a column of Hypersil ODS （4. 6 mm × 250 mm, 5 μm）, using acetonitrilephosphoric acid solution （0.05% ,V/V） as the mobile phase in gradient elution. The flow rate was 10 mL/ min, detection wavelength was 230 nm and 275 nm respectively, and the temperature was 25℃. Results Paeoniflorin showed a good linear relationship at range of 0. 208 μg - 2.08 μg （ r = 0. 999 9 ）. The average recovery was 96. 3 %. Pentagalloylglucose showed a good linear relationship at range of 0. 04 μg- 0. 4μg （r =0. 999 8）. The average recovery was 98.9 %. Conclusion The method is simple, convenient, accurate and sensitive, which can be used for the quality control of Paeonia lactiflora Pall.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2009年第2期122-124,共3页
Journal of Beijing University of Traditional Chinese Medicine
基金
北京工业大学博士启动基金资助项目(No.52015998200504)
