摘要
研究解偶联蛋白-2基因启动子常见-866G/A基因多态性(UCP2-866G/A)与2型糖尿病发病相关性。用多聚酶链反应-限制内切酶长度多态性技术检测了76例非糖尿病对照(NDM)和115例糖尿病患者(DM)的UCP2-866G/A基因型分布,并分析各基因型与胰岛功能、代谢参数的差异性。结果DM的AA基因型分布显著高于NDM(32.2%vs15.8%,χ2=6.526,P<0.038)。在NDM组GG型携带者空腹C肽(FCP)水平高于AA和GG组(两两比较分别为t=2.99,P=0.005和t=2.229,P=0.03);在DM各基因型之间FCP和餐后2小时C肽(2hCP)情况与NDM对照相似,各基因型混和餐刺激后2hCP差异更加明显。结论为UCP2-866G/A基因多态性与大连地区2型糖尿病发病相关,该基因多态性主要影响胰岛β细胞分泌功能。
This study is to investigate the association of uncoupled protein 2 gene promoter -866G/A polymorphism （UCP2 -866G/A） with the development of type 2 diabetes mellitus. UCP2 -866G/A was determined with polymerase chain reaction-restriction fragment long polymorphism in 115 diabetic （DM） and 76 non-diabetic subjects （NDM）. The frequency of AA genotype in DM than in NDM （32.2% vs. 15.8%,χ^2 =6. 526,P〈0. 038）. In NDM C- peptide levels with AA and GG genotypes were lower than that with GA genotype （compared with GA t= 2.99, P= 0. 005 and t= 2. 229, P=0.03 respectively）. The trends of C-peptide levels （fasting and 2 hour postprandial） in different genotypes of DM were similar to NDM, but the differences were only statically for 2 hour postprandial C-peptide levels. Conclusion The UCP2 -866G/A is related to the development of type 2 diabetes mellitus through its effect on insulin secretion.
出处
《医学与哲学（B）》
2009年第3期50-52,共3页
Medicine & Philosophy(B)
