摘要
目的:从抗氧化的角度探讨灯台树皮水煎液治疗实验性小鼠慢性支气管炎的作用机制.方法:称取200g灯台树皮浓缩水煎液到100mL.将60只昆明种雌性小鼠随机分为6组:对照组、模型组、灯台高、中、低剂量治疗组、桂龙咳喘宁胶囊治疗组,每组10只.除对照组外,各组小鼠烟雾联合氨水刺激,共30d,诱导慢性支气管炎模型.第31日起,对照组、模型组小鼠给予生理盐水灌胃,灯台高、中、低剂量治疗组按照10,5,2.5g/kg,桂龙咳喘宁胶囊治疗组1g/kg灌胃给药,1次/d,共15d.治疗结束后,检测6组小鼠肺组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量,血清中髓过氧化物酶(MPO)含量,检测各组小鼠对免疫器官的影响;应用HE染色观察各组小鼠肺组织病理学变化.结果:与慢性支气管炎模型组相比,灯台治疗组的肺组织匀浆SOD,GSH-Px活力升高(P<0.01或P<0.05),MDA含量下降(P<0.01),血清MPO含量降低(P<0.05);胸腺指数、脾指数均明显升高(P<0.05或P<0.01);HE染色观察到灯台治疗后,慢性支气管炎损伤程度改善.结论:灯台树皮水煎液可对抗机体氧化损伤,增强机体抗氧化酶的活力,改善慢性支气管炎存在的氧化—抗氧化失衡,提高免疫功能,减轻气道炎症反应.
AIM： To investigate the mechanism of Alstonia scholaris in the treatment of chronic bronchitis in mice. METHODS： Extract of Alstonia scholaris （200 g） was boiled down to 100 mL. Mice were divided into 6 groups （ 10 mice in each） at random： model group, the high, middle or low dose Alstonia scholaris groups, Guilong group and the control group. Except for the control group and model group, the other four groups were intervened with high, middle or low dose of Alstonia scholaris, and Guilong respectively. Then to determine Activities of SOD and GSH-Px, MDA contents in lung tissues and MPO contents, and changes of immune organ in serum were determined in each group. Pathological changes were also observed with HE staining under microscope. RESULTS： Compared with those in the model group, the activity of SOD and GSH-Px in lung tissues of the drug intervened groups increased obviously, the contents of MDA and MPO decreased significantly, and thymus index and spleen index increased apparently. HE staining showed the alleviation of injury from chronic bronchitis treated with Alstonia scholaris particles. CONCLUSION： Alstonia scholaris has good effects on mice with chronic bronchitis by enhancing the vitality of antioxidant enzymes, preventing lipid peroxidation, removing oxygen free radicals on the injury body, improving immune function and reducing airway inflammation.
出处
《第四军医大学学报》
北大核心
2009年第6期537-540,共4页
Journal of the Fourth Military Medical University
