摘要
目的探讨姜黄素对应激状态下大鼠胃溃疡的防治作用及机制。方法将40只雄性SD大鼠随机分为:正常对照组、溃疡模型组、姜黄素组、奥美拉唑组,每组10只。采用水浸-束缚方法建立大鼠胃溃疡模型,检测各组胃黏膜溃疡指数(UI)、胃液pH值和壁细胞H+,K+-ATP酶活性,观察其胃黏膜组织病理变化,采用SPSS13.0软件进行统计学分析。结果与正常对照组比较,溃疡模型组UI(35.63±3.34)明显升高,胃液pH值(1.35±0.09)明显下降,壁细胞H+,K+-ATP酶活性(9.45±0.34)mmol/(g.h)明显升高(Pa<0.01),镜下可见胃黏膜出血坏死,溃疡形成。与溃疡模型组比较,姜黄素组UI(15.13±2.42)明显下降,胃液pH值(1.81±0.23)明显上升,壁细胞H+,K+-ATP酶活性(8.13±0.59)mmol/(g.h)明显下降(Pa<0.01),镜下胃黏膜损伤程度较轻,未见溃疡形成。姜黄素组与奥美拉唑组比较无显著差异(Pa>0.05)。胃液pH值和壁细胞H+,K+-ATP酶活性水平呈明显负相关(r=-0.884 P<0.01)。结论姜黄素可有效地减轻大鼠胃溃疡模型的胃黏膜损伤,抑制壁细胞H+,K+-ATP酶活性,减少胃酸分泌,对胃溃疡有良好的防治作用。
Objective To explore the preventive and therapeutic effects and mechanism of curcumin on gastric stress ulcer in rats. Methods Forty male SD rats were divided into 4 groups randomly ： normal control group, ulcer model group, eurcumin - treated group and omeprazole - treated group. Water immersion - restraint stress model was performed, and the gastric mucosa ulcer index （ UI）, gastric juice pH and H ^＋ , K ^＋ - ATPase activity were measured. The structural changes in gastric mucosa were observed by histopathologically. The results were analyzed with the Software of SPSS 13.0. Results Compared with normal control group, the UI （ 35.63 ± 3.34） and H^＋ , K^＋ - ATPase activity （9.45 ± 0.34） mmol/（ g · h ） increased significantly （ Pa 〈 0.01 ）, and pH value （ 1.35 ± 0.09） decreased significantly （ P 〈 0.01 ） in ulcer model group. Gastric mucosal hemorrhage and ulcer were observed under light microscope in ulcer model group. Compared with ulcer model group,the UI（ 15.13 ±2.42） and H ^＋ ,K^＋ - ATPase activity（8.13 ±0.59） mmol/（g · h） decreased significantly（P, 〈0.01 ） ,and pH value（ 1.81 ± 0.23 ） increased significantly（ P 〈 0.01 ） in curcumin - treated group. No gastric ulcer but lower - grade injury were observed. The UI,pH value, H^＋ , K ^＋ -ATPase activity and structural changes in gastric mucosa of curcumintreated group were no significant deviation compared with omeprazole -treated group（ Pa 〉 0.05 ）. The gastric juice pH value and H ^＋ , K ^＋ -ATPase activity demonstrated negative correlation（r = - 0. 884 P 〈 0.01 ）. Conclusions Cureumin can obviously reduce gastric mueosal injury,inhibit H ^＋ , K ^＋ - ATPase activity,reduce gastric acid secretion and it has a good preventive and therapeutic effect on gastric ulcer.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2009年第6期451-453,共3页
Journal of Applied Clinical Pediatrics
