摘要
目的:确定银屑病皮损组织与非皮损组织中微小RNA(miRNA)表达的差异。方法:从寻常型银屑病皮损组织和非皮损组织取材,Trizol法抽提细胞总RNA,Ambion miRNA Isolation Kit进一步分离miRNA;利用基因芯片技术,将细胞miRNA与哺乳动物miRNA芯片杂交,采用Lux-Scan 3.0图像软件和SAM version 2.1进行数据分析。结果:银屑病皮损组织与非皮损组织表达的miRNA中有84个存在着明显差异(fold change>2.0),其中46个低表达和38个高表达,最高差异达36倍,部分miRNAs与细胞增殖关系密切。结论:银屑病皮损组织较非皮损组织中miRNA表达数量更丰富,细胞代谢和增殖更活跃;部分miRNAs可能参与银屑病表皮细胞增殖的分子机制。
Objective： To determine the differential expression of miRNA （microRNA） between the lesional tissue and non- lesional tissue of psoriasis vulgaris. Methods： The total RNAs in the lesional tissue and non- lesional tissue of psoriasis vulgaris patients were extracted by Trizol and miRNAs were further isolated by Panbion＇ s miRNA Iso- lation Kit. Hybridization of cell miRNAs to mammalian miRNA chip was made. Data analysis was proceeded by software of LuxScan3.0 and SAM version2.1. Results： A total of 84 miRNAs were found, including 46 low - expression and 38 over- expression miRNAs. The difference between the highest and lowest expressions was 36 folds. Data analysis revealed that some miRNAs were associated with epidermal cell proliferation. Conclusion： The miRNAs expression is more abundant in the lesional tissue than in the non - lesional tissue of psoriasis, reflecting the active metabolism and rapid proliferation in psoriasis. Some miRNAs may be involved in the pathogensis of psoriasis.
出处
《中国麻风皮肤病杂志》
2009年第3期157-159,共3页
China Journal of Leprosy and Skin Diseases
