摘要
应用免疫组织化学方法,研究了I型胶原(Col I)和热休克蛋白90(Hsp90)在肉鸡胫骨软骨发育不良(TD)不同阶段的变化。将80羽1日龄健康AA肉鸡预饲1周后分为2组。对照组饲以基础日粮;试验组在基础日粮中添加福美双100 mg.kg-1,4 d后继续饲以基础日粮。试验历时23 d。应用抗Col I和Hsp90多克隆抗体对试验第4、9、16、23天的肉鸡胫骨生长板进行免疫组织化学研究。结果表明,在饲喂福美双后第4、第9天,Col I和Hsp90的合成在生长板前肥大区和肥大区软骨细胞都明显增加,而第16、第23天时在TD损伤周边的前肥大区软骨细胞出现。由此推测,Col I蛋白合成增加,可能是由于不能正常钙化的反馈调节所致;而上调表达的Hsp90可能在调节软骨细胞发育周期中发挥重要作用,为进一步认识TD提供了新的思路。
The experiment was conducted to identify the changes of collogen type I （Col I） and heat shock protein 90 （Hsp90） at the different stages of broiler tibial dyschondroplasia. 80 one day-old AA broiler chickens were randomly divided into two groups after being raised seven days. The control group was given a regular diet, whereas the experimental group was given the same diet containing thiram 100 mg ·kg ^-1 for four days, then both of the groups was given the same regular diet. The feeding experiment lasted 23 days. Immunohistochemistry was adopted to detect protein synthesis of Col I and Hsp90 in tibia growth plate at the fourth day, the ninth day, the sixteenth day, the twenty-third day respectively by using Col I and Hsp 90 polyclonal antibody. The results showed that synthesis of Col I and Hsp90 was elevated remarkably in prehy- pertrophie and hypertrophic chondroeytes at the fourth day and the ninth day after being fed thiram diet, but the synthesis of Col I and Hsp90 was identified in prehypertrophie chondrocytes a round TD lesion at the sixteenth day and the twenty-third day respectively. It was the non calcification feedback regulation might have resulted in upregulated Col I in deduced that TD and the increased Hsp90 might play an important role in regulating cartilage cell cycle. As a result, the research provides new insights into understanding the pathogenic process of TD in chickens.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2009年第3期428-432,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(30571370)
