摘要
为研究验证内皮素-1(endothelin1,ET-1)是否参与肉鸡腹水肺动脉高压的发病过程,用低温环境下饲养的肉鸡,腹腔注射ET1受体拮抗剂BQ123,检测几项肺动脉高压指标。结果表明:在处理后7和15d,低温空白对照组和低温生理盐水对照组肉鸡肺动脉压和右心室内压显著低于常温空白对照组(P<0.05),低温+BQ123组与常温对照组相比没有显著差异,而显著低于2个未注射BQ123的低温组(P<0.05);各组之间的红细胞压积(PCV)和腹水心脏指数(AHI)在处理后7d都没有显著性差异,而到14d,低温对照组的PCV和AHI显著高于BQ123处理组和常温对照组(P<0.05)。结果说明ETAR特异性受体拮抗剂BQ123能够预防低温诱发的肉鸡肺动脉高压,保护了肺动脉和右心正常的生理功能。本试验用反证法证明了ET-1参与了肉鸡腹水肺动脉高压的发病过程,而且为肉鸡肺动脉高压综合征提供了新的治疗途径。
The blockade effect of endothelin A receptor on blood pressure in experimental model of pulmonary hypertension was examined. Birds raised in low ambient temperature for 7 or 14 days were injected with antagonist BQ123 in bird abdominal cavity. The pulmonary arterial pressure (PAP), right ventricule pressure (RVP) and related parameters were measured. The results showed that both PAP and RVP under low temperature and saline treatment were significantly lower than those of normal temperature control (P<005). The birds injected with BQ123 (2×04 or 20 μg/kg/day) and raised under lower temperature had similar PAP and RVP values as those grown at normal temperature. However, PAP and RVP values of the BQ123-treated birds were significantly lower than those of the birds without BQ123 treatment (P<005). The birds grown at low temperature for 14 days had significantly higher PCV and AHI than those treated with BQ123 and under normal temperature(P<005), But at 7 days, there was not significant difference in PCV and AHI among all the treatments. It was suggested that ET-1 was associated with pulmonary hypertension andascite development. BQ123 showed some meaning in treating pulmonary hypertension.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2005年第3期52-55,共4页
Journal of China Agricultural University
基金
国家自然科学基金资助项目(30070567
30371063)
博士点基金资助项目(20030019032)
