摘要
利用高能日粮常规饲养大群艾维因肉鸡的方法,获得自然肺动脉高压综合征(PHS)肉鸡病例。应用放 射免疫分析方法,分别测定了2～6 周龄正常组和肺动脉高压(PH)组肉鸡血浆及心、肝、肺、肾等组织中内皮素(endothelin-1,ET-1)的含量,并测定了腹水心脏指数(AHI)和平均肺动脉压(mPAP),同时还应用硝酸还原酶法 测定了血浆中一氧化氮(NO)、一氧化氮合酶(NOS)水平。结果显示:PH组AHI、mPAP极显著高于正常组(P<0.01); 从 3周龄开始,PH组ET-1水平开始显著或极显著高于正常组(P <0.05或 P< 0.01),6周龄时 PH组血浆、肺脏和 肾脏ET-1水平略为下降,但仍极显著高于正常组(P<0.01);AHI、mPAP和ET-1均随肉鸡日龄的增长而升高;组织 中ET-1含量高于血浆,肺组织中ET-1含量明显高于其它几种组织;mPAP变化与血浆ET-1含量变化之间存在显著正 相关(P< 0.05),但与 NO 相关性不显著;两组血浆NO、NOS 水平均随日龄增长而升高,且PH 组 NO、NOS水平在后 期显著高于正常组。本试验结果提示,ET-1、NO 参与了肉鸡PHS的形成和发展过程,并在其中起着重要作用。
Natural PHS broiler cases were got through using hyperalimentation dietary to feed a great dealof commercial broiler chickens. Radioimmunoassay was used to detect endothelin-1(ET-1) in plasma, heart, liver, lung and kidney of normal and pulmonary hypertension (PH) broilers at the age of 2,3,4,5 and 6 weeks. The AHI and mPAP of 3 to 6 weeks were measured as well. At the same time, nitrate reductase was used to detect the level of nitric oxide (NO) and nitric oxide synthase (NOS) in plasma of normal and PH broilers at the age of 3,4,5 and 6 weeks. It was found that, compared with the normal group, the AHI and mPAP of PH group were significantly higher, the trend of increment of them with the days delaying was observed. From 3 weeks of age, the level of plasma and tissue ET-1 of the PH group was higher or significantly higher than that of the normal group. At 6 weeks of age, the level of plasma, lung, kidney ET-1 of PH group decreased slightly, though it was still higher than that of the normal group. The level of ET-1 of tissues was higher than that of plasma and the level of ET-1 of lung was higher than that of other organ's tissues. The level of NO and NOS of the two groups increased with the days delayed, and the PH group was significantly higher than the normal group. Besides, there was a significant positive correlation between mPAP and ET-1, but no significant correlation was found between mPAP and plasma NO of the two groups. It is concluded that ET-1 and NO took part in the formation and development of pulmonary hypertension syndrome (PHS) and played an important role in it.
出处
《中国农业科学》
CAS
CSCD
北大核心
2004年第6期912-916,共5页
Scientia Agricultura Sinica
基金
国家自然科学基金资助项目(30270997)
