摘要
目的设计制造自动控制的长期间断低氧大鼠饲养舱,以建立符合睡眠呼吸暂停综合征(SAS)特征的大鼠模型。方法①由单片机自动控制,通过电磁阀控制供应各气体的流量,使饲养舱内的氧浓度能够在9%～21%的范围内快速地变化。②50只SD大鼠均分为五组,即间断低氧2周组(2H)和4周组(4H)、空气对照2周组(2C)和4周组(4C)及正常对照组(NC)。间断低氧组在密闭的舱中间断性地呼吸低氧气体,90s一次循环,每天8h,每周7d。对照组呼吸空气。结果通过单片机能自动调节医用氮气与氧气的输入,使舱内低氧时氧浓度在9.0%±1.5%,复氧时氧浓度在21.0%±0.5%。大鼠平均肺动脉压:2H组较2C组高18.71%,4H组较4C组高16.87%(P均<0.05);右心室收缩末期压及最大变化速率RVESP、RV+dp/dt和RV-dp/dt:4H组较4C组分别高36.36%、56.35%和55.43%(P均<0.01),4H组比2H组分别高88.85%、19.49%和80.97%(P均<0.01);而2C组、4C组与NC组上述指标各组间均无显著性差异(P均>0.05)。结论该大鼠饲养舱能自动、精确控制舱内氧浓度、循环时间,能复制出比较符合SAS病理生理变化特征的动物模型。
Objective To develop an automatic control oxygen density of intermittent hypoxia cabin and establish a sleep apnea syndrome （SAS） model in rats. Methods （1） Regulated by microcontroller automatically, the supply of the gaseous discharge was controlled by solenoid valve to ensure that the oxygen concentrations in hypoxia cabin could change fleetly within the range of 9% - 21% . （2）Fifty SD rats were divided into five groups equally, intermittent hypoxia 2 weeks group （2H） and 4 weeks group （4H）, air control 2 weeks group （2C） and 4 weeks group （4C）, as well as normal control group （NC）. The intermittent hypoxia groups were breathed hypoxie gas intermittently in sealed hypoxia cabin, 90s a circulation, 8 hours per day, and 7 days per week. Results The inputs of N2 （99.99 % ）and O2 （99.5 % ）were regulated by single-chip computer automatically to keep O2 at 9.0% 1.5% in intermittent hypoxia cabin, while reoxygenation O2 was at 21.0% 0.5%. The the mean pulmonary arterial pressure （mPAP） in 2H group was higher 18.71% than that in 2C group, and 4H group was higher 16.87% than that in 4C group （P 〈 0.05） , while there was no significant difference among 2C, 4C and NC groups. The right ventricle functions （RV ＋ dp/dt,RV-dp/dt and RVESP） in 4H group were higher 36.36%, 56.35% and 55.43% than those in 4C group respectively （P 〈0.01）, and 88.85%, 19.49% and 80.97% than those in 2H groups respectively （P 〈 0.01）, while there was no significantly different among 2C, 4C and NC groups. Conclusion The SAS rat model can be successfully established with the method by which the oxygen concentrations in cabin and cycle time can be regulated automatically and precisely, and the effects is well consistent with the SAS pathophysiology.
出处
《中国比较医学杂志》
CAS
2009年第1期25-29,共5页
Chinese Journal of Comparative Medicine
基金
浙江省医药卫生科学研究基金项目计划(2006B105)
温州市科技计划项目(Y20060082)
