摘要
目的观察戊四氮(PTZ)点燃癫痫对大鼠学习记忆功能的影响及海马神经元钙调素依赖性蛋白激酶Ⅱ(CaMKⅡ)表达的变化。方法腹腔内连续注射PTZ制备点燃癫痫大鼠模型,用Morris水迷宫评测大鼠学习和记忆功能,用免疫组织化学技术观察海马神经元α-CaMKⅡ的表达。结果与对照组大鼠比较,在Morris水迷宫试验中,PTZ组寻找平台的逃避潜伏期显著延长(P<0.05),游泳距离显著增加(P<0.05),穿越平台的次数及在平台象限的游泳距离的百分率显著减少(P<0.05),搜索策略变差,同时伴有海马CA1、CA3区α-CaMKⅡ表达明显减少(P<0.05)。结论PTZ点燃癫痫大鼠学习和记忆功能受损可能与海马CA1、CA3区α-CaMKⅡ表达减少有关。
Objective To observe the effect of pentylenetetrazole （PTZ）-kindled epliepsy on rat＇s learning and memory and the expression of calmodulin dependent protein kinase Ⅱ （ CaMK Ⅱ ） in hippocampal neurons. Methods The rats were injected intraperitoneally with PTZ to establish the kindled models. The changes of behavior were evaluated through Morris water maze and the expression of α-CaMK Ⅱ in hippocampal neurons was observed by immunohistochemistry technique. Results The Morris water maze test showed that the escape latency and the swimming distance in PTZ-kindled rats increased significantly （ P 〈 0. 05 ）. The number of platform crossings and the percentage of swimming time in platform quadrant in PTZ-kindled rats decreased obviously （ P 〈 0. 05 ）, and the efficiency of their search strategy was low. It showed that the ability of learning and memory in PTZ-kindled epliepsy rats was obviously impaired, and the expression of α-CaMK Ⅱ in hippocampal CA1 and CA3 sector significantly decreased （P 〈 0. 05 ）. Conclusion The impaired ability of learning and memory in the PTZ-kindled epliepsy rats might be associated with decreased expression of CaMK Ⅱ in hippocampal CA1 and CA3 sector.
出处
《基础医学与临床》
CSCD
北大核心
2009年第3期254-258,共5页
Basic and Clinical Medicine
基金
河北省自然科学基金(C2007000947)
关键词
癫痫
戊四氮
海马
学习记忆
钙调素依赖性蛋白激酶Ⅱ
epilepsy
pentylenetetrazole
hippocmppus
learning and memory
calmodulin dependent protein kinase Ⅱ
