摘要
目的:研究柞蚕雄蛾提取液对放疗后荷瘤大鼠Th1/Th2平衡的影响及机制。方法:60只雄性Wistar大鼠建立W256荷瘤模型,随机分为模型组、单纯放疗组和实验组即放疗联合蚕蛾提取液治疗组,每组各20只。单纯放疗组和实验组给予常规放疗,5Gy/天,连续照射2天,总量10Gy。照射后10天取大鼠脾脏和胸腺,并计算脏器指数;用流式细胞术(FCM)检测外周血T淋巴细胞亚群变化;用酶联免疫吸附实验(ELISA)测定血清中Th1型细胞因子INF-γ和Th2型细胞因子IL-4含量;用免疫组化检测脾脏中Th1型细胞因子INF-γ、Th2型细胞因子IL-4表达变化。结果:实验组与单纯放疗组比较,荷瘤大鼠脾脏和胸腺指数升高;荷瘤大鼠外周血中CD4+T细胞比例与CD4+T/CD8+T值增加;荷瘤大鼠血清中细胞因子INF-γ含量明显升高,而IL-4含量显著降低;脾脏中INF-γ表达增加,IL-4表达减弱。结论:柞蚕雄蛾提取液可以改善荷瘤大鼠放疗后免疫功能抑制状态,改变荷瘤机体免疫功能,可作为恶性肿瘤传统放疗中的辅助用药。
Objective： To explore the underlying mechanism of the extraction from male zooid of Antheraea pernyi on regulating the imbalance of Th1/Th2 of the rats with W256 sarcocarcinoma after radiation therapy. Methods： Tumor models were established by injecting W256 tumor cells into the 60 Wistar rats subcutaneously. And the rats were randomly divided into 3 groups： the model group （MG）, the radiation group （RG）, and the test/experimental group （TG/EG）. The thymus index, spleen index were used in the study as the parameters to evaluate the effect of extract from male zooid on non-specific and specific immune function of rats with tumor. T-lymphocyte subsets in the peripheral blood were detected by Flow Cytometry（FCM） in the rats. The presence of Interferon- gamma （INF-γ） and interleukin-4 （IL-4） were screened by enzyme-linked immunosorbent assay （ELISA） in order to investigating cell-mediated immune responses in the peripheral blood serum. And the expression of INF-γ and IL-4 in the spleen was evaluated by the immunochemistry straining. Result： The results indicated that the extract from male zooid can raise significantly the thymus index, spleen index of rats with tumor. The number of CD4＋T and the CD4＋/CD8＋ ratio of W256-bearing rats treated with the extract from male zooid increased significantly compared with the radiotherapy group （P〈0.05）. And the level of interferon-gamma （INF-γ） was up-regulated and interleukin-4 （IL-4） was down-regulated. Conclusion： The extract from male zooid of Antheraea pemyi has the effect of reversing radiation-induced immunosuppression and could be regarded as a main assistant agents combined with the traditional radiotherapy for malignant tumor.
出处
《现代生物医学进展》
CAS
2009年第5期821-823,859,共4页
Progress in Modern Biomedicine
基金
山东省自然科学基金资助项目(NoY2006C92)
关键词
柞蚕
雄蛾
放射治疗
免疫功能
大鼠
Antheraea pernyi
Male zooid
Radiotherapy
Th1/Th2 shift
Immune function
