摘要
目的建立西黄丸的HPLC-ELSD指纹图谱的质量评价方法。方法采用Waters C18色谱柱(250 mm×4.6 mm,5μm),以甲醇-0.5%醋酸水溶液为流动相,梯度洗脱,柱温30℃。蒸发光散射检测器的条件为漂移管温度45℃,载气体积流量1.5 L/min。结果建立了西黄丸HPLC指纹图谱共有模式,标定出25个共有峰,有24个共有峰来自乳香,1个共有峰(2号峰)来自体外培育牛黄,西黄丸不同批次之间相似度均大于0.9,采用聚类分析(HCA)、主成分分析(PCA)和特征图谱相似度分析对10批西黄丸HPLC特征图谱进行评价,找出相对差异较大的样品以及对于西黄丸质量影响较大的成分。结论该HPLC指纹图谱结合模式识别可以较好地反映西黄丸的内在质量,为西黄丸的进一步研究提供科学依据。
Objective To establish the quality evaluation of fingerprints of Xihuang Pills by HPLC-ELSD. Methods The chromatography conditions were defined as waters C18 column（250 mm × 4.6 mm, 5 μm）; Mobile phase was methanol-0.5% acetic acid, gradient elution; temperature of column was set at 30 ℃. The ELSD conditions were as follows： the temperature of drift tube was 45 ℃, the gas speed was 1.5 L/min. Ten batches of Xihuang Pills samples were analyzed for similarity analysis（SA）, hierarchical clustering analysis（HCA） and principle component analysis（PCA）. Results The chromatographic fingerprint was completed with 25 recognizable peaks, and the samples with great differences and the compounds with greater impact on the quality were obtained through HCA and PCA. Conclusion HPLC fingerprint combining with pattern recognition could reflect the intrinsic quality to provide a scientific basis for the quality control of Xihuang Pills.
出处
《中草药》
CAS
CSCD
北大核心
2017年第11期2219-2224,共6页
Chinese Traditional and Herbal Drugs
基金
广东省科技计划项目(2016A020226031)
广州市科技计划项目(201607010334)
