摘要
昆虫病原真菌是自然界控制害虫种群的主要生物因子,其研究开发越来越受到重视。但由于存在致死速度慢、防效不稳定、对环境条件要求高等缺点,限制了昆虫病原真菌的应用。近年来,通过基因工程技术对昆虫病原真菌进行遗传改造,提高菌株的致病性和毒力,创造高效、稳定的工程菌株取得了较大进展。对昆虫病原真菌选择标记、遗传转化方法、基因工程改良及应用等方面最新研究进展进行了综述。
Entomopathogenic fungi are the main biological factors of controlling pest population in nature, but its application was limited because of their poor performance in field. With the rapid development of fungal molecular biology and gene engineering, large progress has been made in improving the strain＇ s virulence and creating highly efficient and stable strain. The latest advance of entomopathogenic fungi in genetic engineering was reviewed, mainly including selective markers, some transformation methods, and the application of improved strains.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2009年第3期94-99,共6页
China Biotechnology
基金
国家科技支撑计划资助项目(2006BAD08A18)
关键词
昆虫病原真菌
基因工程
转化方法
菌株改良
Entomopathogenic fungi Genetic engineering Transformation method Strain improvement
