摘要
目的建立测定痔痛安软膏中苦参碱与氧化苦参碱含量的HPLC方法。方法采用Inertsil C18色谱柱(4.6mm×250 mm,5μm);以乙腈-0.025 mol/L磷酸二氢钾-0.025 mol/L十二烷基硫酸钠-水(335∶185∶185∶295)为流动相,流速1.0 mL/min,检测波长205 nm。结果苦参碱、氧化苦参碱分别在42～840μg/mL(r=1.000 0,n=6)、9.6～192μg/mL(r=1.000 0,n=6)范围内呈良好的线性关系;平均回收率分别为99.67%(n=6,RSD=1.63%),98.29%(n=6,RSD=2.11%)。结论该方法简便、灵敏、准确、专属性强,重现性好,可作为痔痛安软膏的质量控制方法。
Objective To establish a HPLC method for the determination of matrine and oxymatrine in Zhitong＇ an Creams. Methods The chromatographic column was the Inertsil C18 column（4.6 mm × 250 mm, 5 μm）.The mobile phase consisted of acetonitrile-0.025 mol/L potassium dihydrogen phosphate-0.025 mol/L sodium lauryl sulfate and water （335：185：185：295）. The flow rate was 1.0 mL/min. The detection wavelength was 205 nm. Results The calibration curves were linear in the ranges of 42 - 840 μg/mL （r=1.000 0, n=6） for matrine and 9.6-192μg/mL（r=-1.000 0, n=6） for oxymatrine. The average recoveries were 99.67 % （n=6, RSD=1.63 %） for matrine and 98.29 % （n=6, RSD=2.11%） for oxymatrine. Conclusion This method is simple, rapid and accurate with a good specificity for the quality control of Zhitong＇ an Creams.
出处
《食品与药品》
CAS
2009年第2期38-40,共3页
Food and Drug
