摘要
利用酶解法制备琥珀酸产生菌的孢子原生质体,对其进行紫外线与He-Ne激光的复合诱变,筛选到了一株突变株MS-23,其琥珀酸产量为1.27g/L,是出发菌株产酸量的20.16倍。将此菌株连续传代5次,具有较好的遗传稳定性。
The protoplasts from spores of a strain producing succinic acid were prepared by enzyme and irradiated by both UV and He-Ne laser. After the compound mutation, a high-yielding strain MS-23 was obtained, whose yield of succinic acid is 1.27 g/L, about 20.16 times of its parent.＇After being propagated for five generations, the mutation strain MS-23 shows a more stable heredity.
出处
《食品科技》
CAS
北大核心
2009年第3期6-10,共5页
Food Science and Technology
基金
山东省自然基金项目(Y2007B54)
