摘要
目的探讨Ⅰ型胶原α2（COL1A2）和骨钙素（OC）基因多态性以及血清钙调节相关激素与氟斑牙关系。方法选取河南省开封市开封、通许2个县高氟病区及非病区8～12岁儿童作为观察对象，并根据氟斑牙患病情况分为氟斑牙患者、病区对照及非病区对照组。利用聚合酶链反应一限制性片段长度多态性（PCR—RFLP）法检测COL1A2、OC基因多态性；放射免疫分析法测定血清钙、降钙素（CT）及OC。结果COL1A2 PvuⅡ基因型分布频率为：氟斑牙患者pp占49．3％（37／75）、Pp占32．0％（24／75）、PP占18．7％（14／75）；病区对照pp占43．5％（30／69）、Pp占52.2％（36／69）、PP占4.3％（3／69）；非病区对照pp占43．8％（42／96）、Pp占40．6％（39／96）、PP占15．6％（15／96）。高氟病区携带COL1A2PP基因型的儿童患氟斑牙的危险性高于病区携带pp基因型的儿童（OR=4．85，95％CI：1．22～19.32）。COL1A2RsaⅠ基因型分布频率：氟斑牙患者rr占50．7％（38／75）、Rr占36．0％（27／75）、RR占13．3％（10／75）；病区对照rr占46．4％（32／69）、Rr占46.4％（32／69）、RR占7．2％（5／69）：非病区对照rr占45．8％（44／96）、Rr占45．8％（44／96）、RR占8.3％（8／96），3组基因型分布比较差异无统计学意义（P〉0．05）。OCHindⅢ基因型分布频率：氟斑牙患者hh占48．0％（36／75）、Hh占34．7％（26／75）、HH占17.3％（13／75）；病区对照hh占43．5％（30／69）、Hh占43．5％（30／69）、HH占13．0％（9／69）；非病区对照hh占47．9％（46／96）、Hh占40．6％（39／96）、HH占11．5％（11／96），3组基因型分布比较差异无统计学意义（P〉0．05）。氟斑牙患者尿氟及血清OC水平明显高于非病区对照（P〈0．05），而氟斑牙患者与病区对照间两指标差异无统计学意义（P〉0．05）。结论高氟病区儿童COL1A2 PvuⅡ多态性与�
Objectives To investigate the relationship between fluorosis polymorphisms in collagen type Ⅰ alpha 2 （COL1A2） and osteocalcin （OC） gene, and serum calciotropic hormone levels. Methods The children between 8 and 12 years of age in Kaifeng and Tongxu cities of Henan Province were chosen to be the object of observation. Accoding to situation of dental fluorosis, they were divided into three groups： dental fluorosis group, non-dental fluorosis group from high fluoride areas, and control group form the control areas. The Pvu Ⅱ and Rsa Ⅰ markers of COL1A2 gene as well as HindⅢ marker of OC gene were genotyped by PCR-RFLP procedure. Calcitonin and osteocalcin levels in serum were measured using radioimmunassays. Results The frequency distribution of COL1A2 PvuⅡ genotype was pp 49.3%（37/75）, Pp 32.0%（24/75）, PP 18.7%（14/75） in children with fluorosis; pp 43.5%（30/69）, Pp 52.2%（36/69）, PP 4.3%（3/69） in children without fluorosis from high fuoride areas; and pp 43.8% （42/96）, Pp 40.6% （39/96）, PP 15.6% （15/96） in the children without fluorosis from control areas respectively. Childrens with the homozygous genotype PP of COL1A2 Pvu Ⅱ had a significantly increased risk of dental fluorosis（OR = 4.85, 95%CI： 1.22 - 19.32） compared to children with the homozygous genotype pp in an endemic fluorosis area. The frequency distribution of COLIA2 Rsa Ⅰ genotype was rr 50.7% （38/75）, Rr 36.0% （27/75）, RR 13.3%（10/75） in children with fluorosis; rr 46.4%（32/69）, Rr 46.4%（32/69）, RR 7.2%（5/69） in children without fluorosis from high fuoride areas, and rr 45.8% （44/96）, Rr 45.8% （44/96）, RR 8.3% （8/96） in the children without fluorosis from control areas respectively. There were no significant differences in the three groups （P 〉 0.05 ）. The frequency distribution of OC Hind Ⅲ genotype was hh 48.0%（36/75）, Hh 34.7%（26/75）, HH 17.3% （13/75） in children with fluorosis; hh 43.5% （30/69）, Hh 43.5% （30/69）, HH 13.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2009年第2期142-145,共4页
Chinese Jouranl of Endemiology
基金
河南省科技攻关项目（524410067）
河南省医学科技攻关（20060374）
关键词
胶原Ⅰ型
氟中毒
牙
降钙素
骨钙素
Collagen type Ⅰ
Fluorosis, dental
Calcitonin
Osteocalcin
