摘要
目的调查I型胶原α2链(COL1A2)PvuⅡ基因多态性在人群中的分布,探讨COL1A2 PvuⅡ基凶多态性与高氟区人群骨组织异常的关系。方法在高氟区和对照区选择观察人群,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测COL1A2 PvuⅡ基因型,X线检查人群骨结构改变情况。结果高氟区人群COL1A2 PvuⅡ基因型PP为20．4％,Pp为37．8％,pp为41．8％;对照区人群PP为10．6％,Pp为42．4％,pp为47．0％。两地区人群基因型分布比较差异无统计学意义(X2=2．674,P>0．05);高氟区和对照区人群骨组织异常阳性率分别为88．0％、45．8％,二者比较差异有统计学意义(X2=15．109,P<0．01)。结论COL1A2 PvuⅡ基因多态性与调查人群骨组织异常无明显关系。
Objective To investigate the distribution of collagen type Ⅰ alpha2 （COL1A2） PvuⅡ genotype in population living in areas with high fluoride and to evaluate the relationship between COL1A2 PvuⅡ gene polymorphism and bone abnormality. Methods Polymerase chain reaction-restriction fragment length polymorphism analysis were used to determine COL1A2 PvuⅡ genotype of people in an area with high fluoride and an area without high fluoride. The changes of bone tissues were measured by dual-energy X-ray absorptiometry. Results The PvuⅡ genotype of PP, Pp, pp of COL1A2 distributed in 20.4%, 37.8% and 41.8% of the population in high fluoride area, and in 10.6%, 42.4%, 47.0% of the population in control area, respectively. There were no significant differences in the two areas （X^2 = 2.674,P 〉 0.05）. Bone abnormality was 88.0% and 45.8% in the two areas respectively, and there was a significant difference between them （X^2 = 15.109,P 〈 0.01）. Conclusions There is no correlation between COL1A2 PvuⅡ gene polymorphism and the bone abnormity in the two areas.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2006年第4期386-388,共3页
Chinese Jouranl of Endemiology
基金
河南省科技攻关课题资助项目(524410067)
关键词
Ⅰ型胶原
基因多态性
氟化物
骨组织
Collagen type Ⅰ
Gene polymorphism
Fluoride
Bone abnormality
