摘要
目的:观察成年ROSA26小鼠中枢神经系统半乳糖苷酶(X-Gal)的表达与分布。方法:应用X-Gal组织化学结合尼氏染色及免疫组织化学双标技术研究β-gal在该嵌合体脑内的表达与分布特征。结果:X-Gal组织化学染色显示在该线系小鼠脑和脊髓中均广泛表达转基因β-gal活性产物,但以灰质为主。X-Gal和尼氏双重染色表明,β-gal在神经元、室管膜细胞和脉络丛上皮细胞中均有表达。免疫组化进一步显示β-gal广泛表达于神经元中,但在星形胶质细胞中则未见表达。结论:ROSA26小鼠中枢神经细胞稳定而广泛的表达β-gal,此转基因模式动物将可用于神经细胞培养、移植及其它相关神经生物学研究。
Objective：To observe the expression and distribution of β-galactosidase in the central nervous system （CNS） of aduh ROSA26 mouse. Methods：X-Gal histochemistry combined with Nissl＇s or immunohistochemistry double-staining was performed to localize β-gal activity in the ROSA26 mouse CNS. Results： X-Gal histochemistry staining showed that transgenic β-gal reaction products were throughout the central nervous system, but mainly located at the grey matter. X-Gal and Nissl＇s double-staining showed that β-gal expressed in neurons, ependymal cells and choroid plexus cells. X-Gal histoehemistry combined with immunohistochemistry further revealed that β-galactosidase was ubiquitous expression in neurons except astrocytes. Conclusion：β-gal is extensively and stably expressed in the ROSA26 mouse CNS. This transgenic line may be useful in nerve cell culture, transplantation and other neurobiological studies.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第3期308-311,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省自然科学基金资助(BK2006576和BK2006232)
