摘要
用ＮＡＤＰＨ－黄递酶（ＮＡＤＰＨ－ｄ）组织化学结合免疫细胞化学双标记方法，对大鼠部分脑区内一氧化氮合酶（ＮＯＳ）分别与５－羟色胺（５－ＨＴ）、酪氨酸羟化酶（ＴＨ）和乙酰胆碱（ＡＣｈ）等共存神经元进行了观察。结果表明在中缝背核（ＤＲ）背外侧区，绝大多数ＮＯＳ神经元与５－ＨＴ共存，但５－ＨＴ神经元仍有一半以上未表现出ＮＡＤＰＨ－ｄ阳性。在脑桥蓝斑（ＬＣ）腹内侧部存在大量ＮＯＳ神经元，其中一小部分与去甲肾上腺素（ＮＡ）能神经元（以ＴＨ为标记）共存。在中脑黑质（ＳＮ）区观察到少量ＮＯＳ神经元散在地分布干大量的多巴胺（ＤＡ）能神经元（以ＴＨ为标记）之中，此区还存在大量ＮＡＤＰＨ－ｄ阳性纤维，但没有观察到ＮＯＳ与ＤＡ共存神经元。在前脑中央隔区（ＭＳ）、Ｂｒｏｃａ斜带核水平支（ｈＤＢ）和垂直支（ｖＤＢ），９２％以上的ＮＯＳ神经元与ＡＣｈ共存，这些共存神经元约占所有胆碱能神经元的４２％。纹状体内也有不少ＮＯＳ神经元，但它们不与ＡＣｈ共存。上述ＮＯＳ分别与５－ＨＴ、ＮＡ和ＡＣｈ等经典神经递质共存的结果，为ＮＯ参与多种递质功能的调节提供了形态学基础。
Using NADPH-diaphorase(NADPH-d)histochemistry combined with immunocytochemistry,we investigated the coexistence of nitric oxide synthase(NOS)with serotonin(5-HT),tyrosine hydroxylase(TH)and acetylcholine(ACh),respectively,in some regions of the rat brain;In the dorsloateral subgroup of the dorsal raphe(DR)nucleus,most of the NOS neurons displayed 5-HT immunoreactive(5-HT-IR),while more than half of the serotoninergic neurons were not NADPH-d positive. A large number of NOS neurons presented in the ventromedial subgroup of locus coeruleus(LC), some of them colocalized with noradrenaline(NA)(TH as the maker).In the substantia nigra(SN),NADPH-d+TH double labelled neurons were not observed,although a few NOS neurons containing only NADPH-d activity were visible. Furthermore,there were lots of NADPH-d positive fibers in the SN.In the medial septum(MS),the horizontal and vertical limbs of the diagonal band of Broca (hDB,vDB),more than 92%of NOS neurons displayed ChAT-IR,while such double labelled neurons consisted only about 42%of the cholinergic neurons. A great number of NOS neurons occurred in the striatum,but no NADPH-d+ChAT double labelled neurons were found.The results that NOS coexists with classical neurotransmitters such as 5-HT,NA and ACh in rat brain provide a morphological basis for that NO participates in the modulation of transmitter functions.
出处
《解剖学报》
CAS
CSCD
北大核心
1996年第2期153-157,共5页
Acta Anatomica Sinica
关键词
一氧化氮合酶
经典神经递质
NADPH-D
组织化学
Nitric oxide synthase
Classical neurotransmitters
Transmitter coexistence
NADPH-diaphorase histochemistry
Immunocytochemistry
Rat
