摘要
嗜水气单胞菌广泛存在于水体环境中,可引起人和动物发病,尤其是引起鱼类的败血症。本文构建了pWSK129-gfp重组载体,并分别导入到嗜水气单胞菌强毒株J-1和无毒株4332中。在荧光显微镜下观察,菌体呈现绿色荧光。稳定性试验表明,Ah4332GFP传至70代,质粒稳定率可达100%;而AhJ-1GFP在同样条件下培养10代后,质粒的稳定率仅为15.1%。绿色荧光蛋白基因的导入为研究嗜水气单胞菌与宿主的相互关系提供了一种简单而直观的方法。
Aeromonas hydrophila is a gram-negative bacterium which is widely distributed in aquatic environments. It has long been known as an opportunistic pathogen for a variety of aquatic and terrestrial animals, including humans. Hemorrhagic septicemia caused by A. hy- drophila is a major infectious disease in aquacuhures. In this paper, the recombinant plasmid pWSK129-gfp was constructed, and then trans- formed into the virulent strain J-1 of A. hydrophila （J-1^GFP） and the avirulent strain 4332 （4332^GFP）, respectively. The transformants have bright green performance under fluorescent microscope. The stability assay showed that after 4332^GFP was passaged for 70 generation times, the stability rate of the recombinant plasmid was up to 100%, but only 15. 1% in J-1^GFP after 10 generation times. Genetically marking of A. hydrophila strains with gfp gene provides a simple and intuitive approach to investigate the interaction between the bacterial species and hosts.
出处
《畜牧与兽医》
北大核心
2009年第3期1-4,共4页
Animal Husbandry & Veterinary Medicine
基金
教育部"新世纪优秀人才支持计划"基金(NCET-07-0440)
江苏省自然科学基金(BK2007155)
关键词
绿色荧光蛋白
嗜水气单胞菌
表达
green fluorescent protein
Aeromonas hydrophila
expression
