摘要
目的了解河南省人免疫缺陷病毒1型（HIV-1）毒株的亚型状况及序列变异、流行特征，分析其传播来源和传播规律。方法对2006--2007年在河南省采集的1287例HIV-1感染者抽取静脉血，用巢式聚合酶链反应（nest．PCR）对单个核细胞中前病毒脱氧核糖核酸的env基因和gag基凶进行扩增，并测定和分析核苷酸序列。结果1287份样本中存在B’、C亚型及BC、AE两个重组亚型，其在所有分析样本巾的比例分别为95．882％（1234／1287）、0．466％（6／1287）、2．875％（37／1287）、0．777％（10／1287）。与国内及国际的参考毒株RL42、C．95in21068、07-BC．CN．97．C54A、0JAE．TH．90．CM240间的离散率分别为（9．327±0．245）％、（5．214±0．183）％、（6．278±0．194）％、（5．332±0．158）％。结论目前河南省艾滋病感染者巾有B’、C亚型及BC、AE两个重组亚型。泰国B’亚型依然占主导地位。B’亚型以既往有偿献血人员为主，BC亚型以性传播为主，AE亚型以性传播及既往献血途径为主，C亚型以性传播为主。
Objective To study the epidemic status of human immunodeficiency virus type 1 （HIV-1） subtypes and sequence variation in Henan thus to explore the characteristics and sources of transmission. Methods HIV-1 env and gag gene were amplified by nested PCR from uncultured peripheral blood mononuclear cells （PBMCs） obtained from 1287 HIV-1 carriers confirmed in Henan from 2006 to 2007. 1287 env and gag genes were obtained from the patients. Results Among 1287 samples, there were 4 HIV-1 strains including subtype B＇, C and recombinant subtype BC and AE, accounting for 95.882% （ 1234/ 1287 ）, 0.466% （ 6/1287 ）, 2.875% （ 37/1287 ）, 0.777% （10/1287） respectively. In comparison with the sequence of the international strains of RL42, C.95in21068, 07-BC.CN.97.C54A, 01AE.TH.90.CM240, the genetic divergence was 9.327% ± 0.245%, 5.214% ± 0.183 %, 6.278% ± 0.194% and 5.332% ±0.158%, respectively Conclusion There were 4 HIV-1 strains including subtype B＇ , C and recombinant subtype BC and AE in Henan with main dominant subtype as subtype B＇ which was closely related to HIV-1 strains of Thailand B＇. The major transmit route in subtype B＇ was through blood donation in the past years while with BC, it was through sexual transmission. The major transmit routes in subtype AE were sexual transmission and blood donation. The major route of transmission in subtype C was through sexual contact.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2009年第3期231-234,共4页
Chinese Journal of Epidemiology
