摘要
目的了解黑龙江省内部分人免疫缺陷病毒1型(HIV-I)的亚型及基因序列特征。方法用巢式聚合酶链反应(nested-PCR),对黑龙江省内17份HIV-1感染者外周血单个核细胞(PBMC)中前病毒脱氧核糖核酸的膜蛋白(e(?))基因进行扩增,并对C2-V3的核苷酸序列进行测定和分析。结果系统树分析显示,17份样本中病毒与HIV泰国B(B′)亚型聚在一起,基因离散率为(6.194±1.01)%,与欧美B亚型基因离散率为(12.94±2.19)%,与其他亚型的离散率大于20%。对于其V3环四肽序列的分析表明,具有GPGQ的8例,占47.06%;具有GPGR的7例,占41.18%;1例为GQGR;1例为GPGH。通过序列分析预测,大部分利用CCR5辅助受体。结论所检测的黑龙江省17例HIV- 1均为B′亚型,提示黑龙江省的HIV-1流行株可能以B′亚型为主,其V3环顶端四肽序列特征以GPGQ和GPGR为主。
Objective To investigate the subtype distribution and sequence characteristics ot HIV-1 strains in Heilongjiang province. Methods HIV-1 env gene was amplified by nested-PCR from peripheral blood mononuclear ceUs（PBMC） obtained from 17 HIV-1 seropositive individuals in Heilongjiang province. The C2-V3 region was sequenced. Results Phylogenetic tree analysis showed that 17 HIV strains clustered closely to Thailand B（ B＇ ） and were far different from other international subtypes. The gene divergence of 17 samples is （6.94 ±1.01 ）% with reference strains of Thailand B（B＇） subtype, （12.94 ± 2.19）% with Europe and America B, over 20% with other subtypes. The analysis of V3 loop amino acid sequence of 17 HIV strains revealed that V3 tip motif of 8 samples （47.06%） were GPGQ, 7 samples （41.18%） were GPGR, 1 sample was GQGR and 1 sample was GPGH. Most cases could utilize CCR5 coreceptor through forecasting V3 sequence. Conclusion The findings suggested that the epidemic subtype of HIV-1 in Heilongjiang province was mainly B＇, V3 tip motifs of the HIV-1 isolates were mainly GPGQ and GPGR.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2007年第7期619-622,共4页
Chinese Journal of Microbiology and Immunology
基金
黑龙江省政府博士后基金(LRB05-263)
黑龙江省卫生厅科学技术研究一般项目(2005-172)
