摘要
目的探讨人胚胎发育早期脊髓内细胞凋亡的变化规律。方法应用脱氧核糖核酸末端转移酶介导的缺口末端标记(TUNEL)法检测和图像分析(NIS-DR)软件测量第2、3、4三个月龄段,人胚胎脊髓组织中凋亡细胞的积分光密度(IOD)值,数据的组间比较采用单因素方差分析。结果第2、3、4三个月胎龄段,人胚胎脊髓中央管、前角和后角处均有凋亡细胞存在。2个月胎龄组脊髓组织中,TUNEL阳性细胞的IOD值为134.9954±10.5326(n=4),3个月组IOD值为149.0331±5.6619(n=5),4个月组IOD值为140.7892±7.6532(n=5),可见IOD值有先升高后降低的趋势,三组比较差异有统计学意义(P<0.01)。结论细胞凋亡在人胚胎脊髓的生长发育过程中起重要的调节作用。
Objective To explore the regularity of cell apoptosis in the early development of human embryonic spinal cord tissue Methods The apoptotic cells in the spinal cord tissue of human embryos were stained in the second, third and fourth month of gestation by terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling （TUNEL） method, and the integral optical density （IOD） was analyzed with Nikon imaging system （NIS-DR）. One-Way ANOVA was applied to compare these IODs. Results The positive expression of cells apoptosis was detected in the central canal, anterior horn and posterior horn of human embryonic spinal cord in the second, third and fourth month of gestation. The IOD values of apoptotic ceils at the 3 time points were 134. 9954 ± 10. 53257, 149. 0331± 5. 66187 and 140. 7892±7. 65320, respectively. The IOD value of apoptotic cells of human embryonic spinal cord at the third month of fetal age was higher than that of the second and the fourth month of fetal age, and the IOD value at the fourth month of fetal age was higher than that of the second month of fetal age. With the advancement of fetal age, the expressions of 1013 of apoptotic cells showed an up then down trend. One-Way ANOVA was employed to compare the IOD values of the apoptotic cells detected in the 3 fetal periods, and the results showed significant difference （P〈0. 01）. Condusion Cell apoptosis plays an important role in regulating the development of human embryonic spinal cord tissue
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2009年第3期315-317,共3页
Medical Journal of Chinese People's Liberation Army
