摘要
目的:探讨淋巴细胞CD4、CD69和细胞因子RANTES、IL-5、IL-8在鼻息肉组织中的表达及其在鼻息肉发生、发展中的作用。方法:苏木精-伊红染色、免疫组织化学和图像分析法检测34例鼻息肉组织(鼻息肉组)和30例鼻甲黏膜组织(对照组)中CD4、CD69、CD34、RANTES、IL-5、IL-8的表达。结果:鼻息肉组中淋巴细胞、嗜酸粒细胞弥漫浸润,腺体增生、杯状细胞增生、纤维增生、间质水肿的阳性率明显高于对照组(均P<0.01)。鼻息肉组中CD4、CD69、IL-5、IL-8、RANTES阳性淋巴细胞数密度(n/mm2)明显高于对照组(均P<0.05);CD4、CD69和RANTES、IL-5、IL-8的表达呈显著正相关(P<0.01、P<0.05和P<0.05)。IL-5、RANTES的高表达分别与嗜酸粒细胞浸润呈显著正相关(P<0.05、P<0.01)。IL-8的表达和鼻息肉组织中微血管形成呈显著正相关(P<0.01)。结论:CD4、CD69过度活化的T淋巴细胞及其产生的相关细胞因子IL-5、IL-8、RANTES可能促进和形成鼻息肉的免疫发病过程;IL-5、RANTES可促进嗜酸粒细胞的浸润、聚集、活化;IL-8可促进微血管形成。
Objective：To investigate the expression of CD4, CD69, CD34, RANTES, IL-5 and IL-8 in nasal polyp tissues, and study their roles in the formation of nasal polyp. Method：The expression of CD4, CD69, CD34, RANTES, IL-5 and IL- 8 were detected by immunohistochemical method and image analysis in 34 cases of nasal polyps and 30 cases of nasal concha mueosa （LNT）. Result：The positive rate of grandular organ hyperplasy, forma tion of beaker cell, fiber heperplasy,interstitial edema and infiltration of lymphocyte and eosinophilic granulocyte in nasal polyps were significantly higher than those in nasal eoncha mucosa （P〈0. 01）. The cell density （piece/ mm^2 ） of CD4^＋ , CD69^＋ , IL-5, IL-8, RANTES in 34 nasal polyps was significantly higher than those I nasal con cha mucosa （P〈0.05）. Marked positive correlations were found between expression of CD4, CD69 and RANTES, IL-5 and IL-8 （P〈0.05, P〈0.01 and P〈0.05）, expression of IL- 5 and RANTES and infiltration level of eosinophilic granulocyte （P〈0. 05 and P〈0. 01）, and expression of IL-8 and vasoformation on nasal polyps tissue （P〈0.01）. Conclusion： T lymphocytes and correlated cytokines participate in the immunopathogenesis of nasal polyps; IL-5 and RANTES can prompt the infiltration.the aggregation and the activation of eosinophilic granulo cytes; IL-8 can promote the vasoformation in nasal polyps.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2009年第5期201-204,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
