摘要
目的探讨脑出血后脑水肿和MMP-9表达的动态变化及依达拉奉的干预效应。方法采用立体定向技术制作大鼠脑出血模型,干湿重法测量脑组织含水量和免疫组化法测定脑组织MMP-9的表达。结果与假手术组比较,脑出血12h时脑组织含水量明显增加(P<0.05),72h时达到高峰,随后逐渐下降,7d时仍高于假手术组(P<0.05)。出血组大鼠脑含水量与血肿周围MMP-9阳性细胞数呈正相关(r=0.846,P=0.034),且差异有统计学意义。(3)治疗组脑含水量和MMP-9阳性细胞数与假手术组比较差异有统计学意义,与出血组比较差异亦有统计学意义(P<0.05)。结论MMP-9参与了脑出血后脑水肿的形成,依达拉奉对脑水肿有抑制作用。
Objective To study the relationship between brain water content and the brain tissue expression of MMP-9 in rat models of experimental ICH and the intervention of edaravone. Methods ICH rat model was made by stereotaetic technique. We measured brain water content, and MMP-9 expression with immunohistochemistry after ICH. Results The brain edema and MMP-9 expression reached the maximum after ICH from 12h to 72h. There were marked differences compared with the sham. The MMP-9 expression was positively correlated with the brain water content （r= 0. 846, P = 0. 034）. Brain water content and the brain tissue expression of MMP-9 of treatment group were higher than other groups. The differences were marked. Conclusion The increase paralled that of the amount of haemorrhage and brain water content. It was postulated that MMP-9 enhanced development of brain edema through degrading of the blood brain barrier component substances after ICH. Edaravone can protect brain tissue by inhibiting brain edema.
出处
《中国实用神经疾病杂志》
2009年第3期31-34,共4页
Chinese Journal of Practical Nervous Diseases
