摘要
通过小鼠每日颈背皮下注射D-半乳糖来研究小鼠衰老模型的制作及效果评价。取昆明种小鼠按体质量随机分空白组、D-半乳糖组、阳性对照组。颈背皮下注射D-半乳糖，连续给药，灌胃60d后，测定胸腺和脾脏指数、小鼠血清中超氧化物歧化酶（SOD）。模型组小鼠胸腺和脾脏萎缩，血液中SOD活力显著降低。颈背皮下注射D-半乳糖小鼠衰老模型可作为衰老及抗衰老实验动物模型使用。
OBJECTIVE To study the making of senile model and the evaluating of the effects by injecting D-galactose into the nape of all mice every day. METHODS Random groups were prepared by weight for three groups, which are one bland group, one model group and one masculine group . Inject D-galactose into the nape of all mice and give medicine for 60 days before testing the thymus and spleen index and superoxide dismutase activities in blood. RESULTS The thymus and the spleen of the model group were atrophied and the vitalities of superoxide dismutase in blood was increased obviously. CONCLUSION The effects of injecting D-galactose into the mice is better and can make the senile model and can be appied to anti-aging experiments in mice.
出处
《武汉工业学院学报》
CAS
2009年第1期32-33,36,共3页
Journal of Wuhan Polytechnic University
