摘要
[目的]探寻黄连木的组织培养繁殖技术，为进一步开展品种改良奠定基础。[方法]以2年生嫁接苗茎段为外植体，通过诱导腋芽进行启动培养试验。分别研究不同灭菌剂与灭菌时间、不同抑制剂以及不同光照条件等对黄连木组培效果的影响。[结果]试验初步得出，用0．1％升汞灭菌黄连木外植体较合适的时间为8～12min，半木质化茎是最合适的诱导腋芽的茎段；对于抑制褐变方面，使用聚乙烯吡咯烷酮（PVP）相对较好些；而在培养初期，光照强度对腋芽的诱导情况影响不大。[结论]黄连木组织培养过程中极易出现褐变现象，而且污染率也很高，生产中应注意采取适当的技术措施避免该现象。
[ Objective] The technique of Chinese pistache tissue culture propagation was explored in order to furher lay the foundation for variety improvemerit. [ Method] The experiment in the axillary induction with 2-ycar-old grafted seedlings as explants was conducted.The effect of different sterilizations, sterilized times, inhibitors and light conditions on Chinese pistache tissue was studied. [ Results ] The preliminary result indicated that the suitable time of explants＇ sterilization with 0. 1% mercuric chloride was 8 - 12 rain and semi-lignified stem was the most appropriate explants for axillary induction. The use of PVP had relatively good effect on tissue brown-inhibiting. In the early days of material-culturing, the light intensity had little effect on the axillary induction. [ Conclusion] The tissue-browning in the process of Pistacia tissue culture was easily, and also, there was high pollution rate, so some appropriate measures should be taken to avoid the phenomenon in production.
出处
《安徽农业科学》
CAS
北大核心
2009年第3期974-975,共2页
Journal of Anhui Agricultural Sciences
关键词
黄连木
启动培养
褐变
污染率
Pistacia
Starting culture
Browning
Pollution rate
