摘要
目的:研究大黄素对小鼠皮肤移植的免疫抑制作用,探讨其可能的作用机制.方法:建立小鼠C57BL/6→BALB/c异体皮肤移植模型.以大黄素和环孢素A(CsA)分别灌胃,观察不同药物及药物浓度对小鼠皮肤移植物存活时间的影响.采用ELISA法检测大黄素和CsA对皮肤移植小鼠血浆IL-2表达水平的影响.组织学观察不同药物浓度对移植物病理变化的影响.结果:大黄素显著降低小鼠血浆内IL-2的浓度,同时伴有移植物的存活时间的延长.组织学观察发现大黄素可以减轻移植排斥反应的强度,其作用表现出量效依赖性,并与CsA具有协同性.结论:大黄素能够减轻C57BL/6→BALB/c异体皮肤移植的排斥反应强度,其作用机制可能与抑制IL-2的分泌有关.
AIM： To investigate the immunosuppressive effects of emodin on skin transplantation in mice and its immunosuppressive mechanism. METHODS ： C57BL/6→BALB/c skin trans-plantation models were established and then treated with different concentrations of emodin and cyclosporine A （CsA）. The different mean survival times （MST） of different groups were observed. Changes of plasma cytokines （IL-2） in mouse skin transplantation models were determined by ELISA and histological changes at different concentrations of emodin and eyclosporine A （CsA） were investigated. RESULTS： In the group treated with emodin, the MST of skin graft was prolonged and production of plasma IL-2 decreased. The histological results suggested that emodin relieved the graft immunological damage in a dose-dependent manner and exerted synergistic effect with CsA. CONCLUSION： Emodin exerts some immunosuppressive effects on mouse skin transplantation possibly by suppressing IL-2.
出处
《第四军医大学学报》
CAS
北大核心
2009年第4期301-304,共4页
Journal of the Fourth Military Medical University
基金
新世纪优秀人才支持计划资助(NECT)
