摘要
以一株分离自高原掌叶大黄的腐皮镰刀菌R13为研究对象,为进一步研究实验室条件下提高该菌生产大黄素产量及相关的合成途径,研究采用了调节初始发酵pH及添加蒽醌合成途径前体物质的方法,并通过HPLC对大黄素进行定量分析.研究发现添加丙酸、丙二酸时对该菌生产大黄素具有促进作用并表现出一定的浓度依赖效应,可以初步确定该菌生产大黄素的合成途径为聚酮合成途径;经优化该菌生产大黄素的产量达到3.908mg/L.
The Fusarium solani R13, isolated from Rheum palmatum L. lived in plateau, was studied inthis research. In order to raise the emodin production of R13 and explore the synthetic route of emodin,the ways of regulating the initial pH of the fermentation broth and adding precursors of the anthraqui-none synthetic pathway were adopted, and the quantitative analysis was made by using of HPLC. Theresult showed that the production of emodin was promoted when adding propionic acid and malonic acid,and the effects were concentration dependent. It could be preliminary inferred that the synthetic pathwayof emodin produced by F. solani R13 belonged to anthraquinones biosynthesis route. And the productionof emodin achieved 3. 908 mg/L, which lay the foundation for practical application of emodin producedby F. solani R13.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2015年第5期1135-1140,共6页
Journal of Sichuan University(Natural Science Edition)
基金
四川省科技支撑计划(2012SZ20045)
国家科技支撑计划(2009BAI84B01)
关键词
腐皮镰刀菌
大黄素
蒽醌
合成途径
掌叶大黄
Fusarium solani
Emodin
Anthraquinone
Synthetic pathway
Rheum palmatum L.
