摘要
目的研究几种基因的异常在肺癌过程中所起的作用。方法采用免疫组化、ＤＮＡ／ＰＣＲ直接测序及ＲＴ－ＰＣＲ方法对５９例原发性肺癌组织ｐ５３、ｍｙｃ基因和ｍｄｒｌ基因进行检测。结果在肺癌组织中ｐ５３基因突变或过表达占６５％（３７／５７），ｍｙｃ基因过表达占５９％（２７／４６），ｍｄｒｌ基因过表达占３１％（１５／４８）。其特点是：ｐ５３基因异常与肿瘤大小、淋巴结转移、病期和复发无相关性，而ｍｙｃ基因的过表达与之呈正相关，ｍｄｒｌ基因过表达在腺癌中占有较高比例，它与淋巴结转移及病期无相关性。ｐ５３和ｍｙｃ二者均异常者占６３％（１９／３０），其复发率为７６％，ｍｄｒｌ和ｍｙｃ均异常者为６２％，其复发率为８３％。结论ｐ５３、ｍｄｒｌ与ｍｙｃ基因的异常在肺癌中可能具有协同作用。
Objective To study alteration of several genes in the process of primary lung cancer.Methods A series of 59 lung cancer specimens were analyzed for p53, myc oncogene family and mdrl gene by DNA/PCR sequencing, immunohistochemistry and RT PCR methods.Results p53 mutation or/and protein accumulation were found in 37 of 57(65%) cases. Overexpression of myc family oncogenes and mdrl gene were 27/46 (59%) and 15/48 (31%), respectively. The results also showed that there was no significant correlation between p53 alteration and tumor size, metastasis, stage and relapse, but there was a significant correlation between overexpression of myc family oncogene and these factors. Overexpression of mdrl gene was detected in NSCLC, especially in adenocarcinoma, and was not associated with metastasis and stage of lung cancer. It was also found that aberration of both p53 and myc family oncogenes occurred in 19/30(63%) cases; the relapse rate was 76%. Both overexpression of mdrl and myc gene was 62%; the relapse rate was 83%.Conclusion p53, myc and mdrl genes in cooperation may be involved in the process of lung cancer, but prognostic determinant is myc gene overexpression.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
1998年第1期23-25,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
