摘要
目的:研究肺癌与正常肺组织穿刺标本中端粒酶催化基团mRNA表达与肿瘤分期与分级关系,探讨其在肺癌早期诊断中的临床意义。方法:采用原位杂交技术检测肺癌与正常肺组织穿刺标本中端粒酶催化基团mRNA表达。结果:30例肺癌穿刺标本中28例端粒酶催化基团mRNA表达阳性,阳性率93.3%;对照组30例中端粒酶催化基团mRNA表达阳性1例,阳性率3.3%。表达与肿瘤分期与分级无关。结论:肺癌穿刺标本中端粒酶催化基团mRNA在肺癌中有异常阳性表达,但其表达与肿瘤分期与分级无关。
Objective To evaluate the expression of human telomerase reverse transcriptase messenger RNA（ hTRT mRNA）in lung cancer tissue samples and the relationship with tumor stages and grades. Methods Expression of hTRT were investigated with in situ hybridization of biopsy specimens from 30 cases of lung cancer and 30 cases of benign lung disorders. Results Of the 30 cases of lung cancer, 28（93.3% ） cases expression hTRT mRNA, while only 1 （3.3%） specimen from benign lung disorder cases showed morderate expression of hTRT mRNA, The expression level of hTRT mRNA in cancer was significantly higher than that of non - cancer tissue （P 〈 0.01）. The expression level of hTRT mRNA had no correlation with the disease stage （P 〉 0.05 ）. Conclusion Lung cancer tissues had increased positive expression of hTRT mRNA, yet the expression had no relationship with tumor stages and grades.
出处
《放射免疫学杂志》
CAS
2009年第1期71-72,共2页
Journal of Radioimmanology
基金
沈阳市科学技术基金:项目编号:1053125-1-49
关键词
肺癌
端粒酶
原位杂交
lung cancer, telomerase, in situ hybridization
