摘要
目的研究非酒精性脂肪性肝病(NAFLD建模成功后行大网膜切除术,大鼠血清ALT、AST、TG、FFA、脂联素浓度变化的影响。方法Wistar大鼠54只,随机分为三组,正常对照组(A组)、高脂饮食组(B组)及高脂饮食联合大网膜切除组(C组);检测各组大鼠血清三酰甘油(TG)、游离脂肪酸(FFA)、丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)及脂联素浓度变化。结果大网膜切除后血浆中脂联素水平较单纯高脂饮食组明显升高(P<0.05),在12周末,C组血清FFA、TG浓度较B组明显降低(P<0.05)。结论大网膜切除可明显提高NAFLD大鼠血清中脂联素水平,并改善血生化指标,提示内脏脂肪在NAFLD发病过程中起重要作用,可能通过脂联素参与NAFLD的发病机制。
Objective To investigate the effect of ALT,AST,FFA,TG and adiponectin in serum and in NAFLD rat model with greater omentum resection. Methods Fifty-four Wistar rats were randomly divided into 3 groups：group A, control group; group B, fat-riched diet group; group C,greater omentum resection in 8 weeks later fat-riched diet. The serum concentration of FFA, TG, ALT and AST were measured with biochemical method in rats. The surum concentration of adiponectin was measured by enzyme immunoassay. Results Compared with the group C, the content of serum FFA and TG in group B significantly increased. At the end of 8th week,the surum concentration of adiponectin increased in guoup C compared with guoup B （P^0.05）. Conclusion The suture concentration of adiponectin increased in group C as well as the blood^biochemical indicators improved, showing that visceral fat could play important role in NAFLD and participate in pathological process of NAFLD through adiponectin.
出处
《重庆医学》
CAS
CSCD
北大核心
2009年第4期392-394,共3页
Chongqing medicine
基金
重庆市自然科学基金资助项目(CSTC2007BB5074)。
关键词
大网膜切除术
非酒精性脂肪性肝病
脂联素
内脏脂肪
greater omentum resection
non-alcoholic fatty liver disease
adiponectin
visceral fat
