摘要
以马链球菌为出发菌株,通过紫外线和60Co-γ射线辐照诱变,得到一株无溶血性菌株NC1150,并在此基础上继续用60Co-γ射线辐照诱变得到产量较高的菌株NC168,使透明质酸产量与出发菌株NC1150相比提高了101%,相对分子量为0.55×106D,突变株经过多次传代,透明质酸产量和相对分子量保持稳定,溶血性无回复突变现象。
In this study, a mutant designated NC1150 which dose not produce streptolysin was selected from wild-type Streptococcus equisimilis by ultraviolet ray combined with ^60Co-y ray mutation. Through further ^60Co-y ray mutation a high-producing hyaluronic acid mutant designated NC 168 was obtained. This selected mutant NC 168 could produce hyluronic acid 1.01 times as much as the original strain NC 1150 and relative molecular weight of hyaluronic acid touched 0.55×10^6 D. Furthermore, its hyaluronic acid output and rela- tive molecular weight of hyaluronic acid maintained genetic stability after 10 generations and there was no reverse mutation on hemolysis.
出处
《微生物学通报》
CAS
CSCD
北大核心
2009年第2期205-210,共6页
Microbiology China
关键词
马链球菌
透明质酸
紫外线诱变
^60Co-γ射线诱变
Streptococcus equisimilis, Hyaluronic acid, Ultraviolet ray mutation, ^60Co-7 ray mutation
