摘要
目的研究T2DM患者线粒体ND1基因3537A→G和ND2基因4824A→G、5351A→G突变与T2DM的相关性。方法应用PCR-RFLP技术检测145例T2DM和334例正常对照者(NC)线粒体DNA(mtDNA)3537A→G、4824A→G、5351A→G突变情况。结果 NC组mtDNA 4824A→G突变率高于T2DM组(P<0.05)。3537A→G及5351A→G突变率在两组中无统计学差异(P>0.05)。结论 mtDNA 4824A→G突变可能为T2DM患病的保护因素。3537A→G及5351A→G突变可能与T2DM不相关。
Objective To evaluate the association between mutations of mitochondrial DNA at 353TANG, 4824A→G, 5351A→G and type 2 diabetes mellitus in Dalian area. Methods PCR-RFI.P was used to detect the 353TANG, 4824A→G, 5351A→G mutations of mitochondrial DNA（mtDNA） in 145 patients with type 2 diabetes mellitus and 334 non-diabetes subjects with normal glucose tolerance without family history of T2DM in Dalian area. Results The prevalence of mitochondrial DNA 4824A→G mutation was lower in T2DM group than in control group[4.1% （6/145）][10.20% （34/334）]（X^2= 4.82, P〈0.05）. However the prevalence of 3537A→G[1.4% （2/145） vs 4.2% （14/334）] and 5351A→G mutation [4.8% （7/145） vs 2.1% （7/334）] did not reach a significant difference between the two groups （P〉0.05）. Conclusions The mtDNA mutation of 4824A→G may be a protective factor for the development of type 2 diabetes mellitus.
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2009年第2期114-115,共2页
Chinese Journal of Diabetes
基金
国家重点基础研究发展规划973计划资助项目(2006CB503901)
国家人类遗传资源共享平台建设资助项目(2006DKA21301)
关键词
糖尿病
2型
线粒体DNA
突变
Diabetes mellitus,type 2
Mitochondrial DNA
Mutation
