摘要
为了解浙江省温州地区2型糖尿病病人中线粒体DNAtRNALeu(UUR)基因A3243G及NADH脱氢酶亚单位1(ND1)基因G3316A位点突变的发生频率,并探讨突变与2型糖尿病主要临床指标出现的相关性。对随机收集的无血缘关系的244例温州地区2型糖尿病患者进行研究,同时选择156例无DM家族史的糖耐量正常者作为对照组,用聚合酶链反应及限制性片段长度多态性分析技术进行点突变筛选,筛选到的异质性突变样本经T-A克隆后再作测序和变性高效液相色谱(DHPLC)确证。结果在244例的2型糖尿病患者中检出A3243G突变1例(0.410%),156例对照者中未检出该突变,突变发生率在两组间差异无统计学意义(P>0.05);2型糖尿病患者中检出G3316A突变4例(1.639%),156例对照者中检出突变2例(1.282%),突变发生率在两组间差异无统计学意义(P>0.05)。结果表明线粒体tRNALeu(UUR)基因A3243G突变在浙江温州2型糖尿病人群中发生频率低,不是温州人群中2型糖尿病的常见病因。线粒体ND1基因G3316A突变在糖尿病人群中的发生频率也较低,且在正常人群中也有出现,可能仅为人群中线粒体DNA的基因多态性。
To investigate the frequencies of mitochondria DNA （mtDNA） tRNALeu （UUR） point mutation A3243G and NADH dehydronase subunit I（ND1） gene point mutation G3316A in Wenzhou area of Zhejiang Province, and to explore the correlation between these mutations and the clinical manifestations in patients with type 2 mellitus diabetes（T2DM）. Two hundreds and forty-four unrelated patients with T2DM and 156 healthy subjects without family history of T2DM were enrolled in Wenzhou area in this study and screened for the point mutations mentioned above with polymerase chain reaction （PCR） and restricted fragment length polymorphism（RFLP） analysis. The heterogeneous mutations were confirmed with DNA sequencing and denaturing high performance liquid chromatography （DHPLC） following T-A cloning of PCR products. The percentage of A3243G mutation in group of patients with T2DM and control were 0.410% and 0.0% （1/244 vs 0/156）, respectively; however, there＇s not any significant difference between these two groups in frequency of A3243G mutation （P〉0.05）. G3316A mutation was detected in 4 of 244 cases with T2DM （1.639%） and 2 of 156 healthy controls （1. 282%）, showing that there＇s also no statistic difference between these two groups in frequency of G3316A mutation （P〉0.05）. It＇s shown that the frequency of mtDNA tRNA^Leu （UUR） A3243G mutation is fairly low in patients with T2DM in Wenzhou area. Thus it＇s reasonable to assume that this mutation may not be involved in the development and progression of T2DM. Furthermore, it＇s demonstrated that the rate of G3316A mutation of mtDNA ND1 gene is rare in patients with T2DM in Wenzhou area and this mutation also happened in healthy control. It＇s suggested that G3316A mutation is just a gene polymorphism of mtDNA and not related to the pathogenesis of T2DM.
出处
《遗传》
CAS
CSCD
北大核心
2006年第10期1206-1212,共7页
Hereditas（Beijing)
基金
国家自然科学基金资助项目(编号:30470958)
温州市科技局项目(编号:Y2005A029)~~
关键词
2型糖尿病
线粒体DNA
点突变
多态性
type 2 diabetes mellitus
mitochondrial DNA
point mutation
polymorphism
