摘要
目的:探讨内皮素-1(ET-1)对大鼠骨髓内皮前体细胞(EPCs)凋亡及细胞周期的影响.方法:采用密度梯度离心法获取大鼠骨髓单核细胞,在M199培养液中培养扩增EPC并进行鉴定.EPCs被分为对照组和ET-1组(100μmol/L),各组再分为不同干预时间组(24,48,72,96h),流式细胞仪检测EPC凋亡率和细胞周期比例.结果:24,48,72,96hET-1组凋亡率均比相应时段对照组低(P<0.01);72hET-1组和96hET-1组分别较48hET-1组有所降低(P<0.05和P<0.01).24,48,72,96hET-1组G0/G1期细胞比例均较相应时段对照组减少(P<0.01);24,48,72,96hET-1组S期细胞比例均较相应时段对照组增加(P<0.01);24,48,72,96hET-1组G2/M期细胞比例均较相应时段对照组增加(P<0.01).结论:ET-1可以同时促进EPC凋亡和增殖,随着干预时间延长,增殖效应逐渐显著.
AIM： To study the effects of endothelin-1 （ ET-1 ） on apoptosis and cell cycle of endothelial progenitor cells （EPC） from rat bone marrow. METHODS： Mononuclear cells were collected from rat bone marrow by density gradient centrifugation, cultured with Medium 199, and were identified by Di Ⅰ -acetylated low- density lipoprotein（Di Ⅰ-ac-LDL） and FITC-ulex europaeus agglutinin-Ⅰ （FITC-UEA-Ⅰ ） double staining. EPCs collected at 5th day of culture were divided into 2 groups： Control group and ET-1 group （ 100 μmol/L）, then were continuously incubated for different periods（24, 48, 72, 96 h）. The EPCs apoptosis and cell cycle were analyzed by flow cytometry （FCM）. RESULTS： The EPCs apoptosis rate was higher in ET-1 group than in control group at 24, 48, 72, 96 h（P 〈 0. 01 ）. Compared with 48 h ET-1 group, the EPCs apoptosis rate decreased in 72 h and 96 h ET-1 groups （P〈0.05, P 〈 0.01）. The amount of EPCs in 24 h, 48 h, 72 h, 96 h ET-1 groups in G0/G1 phase were significantly lower than those of control group（P 〈 0. 01 ）. Compared with the 24 h, 48 h, 72 h, 96 h control group, the amount of EPCs in 24 h, 48 h, 72 h, 96 h ET-1 groups in S phase and in G2/M phase were significantly increased（ P 〈 0.01 ）. CONCLUSION： ET-1 can significantly stimulate both the proliferation and apoptosis of EPCs. With the prolongation of intervention duration, the impact on the proliferation becomes more prominent.
出处
《第四军医大学学报》
北大核心
2008年第22期2026-2028,共3页
Journal of the Fourth Military Medical University
基金
国家自然科学基金重点项目(30600580)
