摘要
目的通过建立SD大鼠MODS模型,观察肠功能恢复汤对多器官功能障碍综合征(MODS)大鼠细菌和内毒素移位及血清丙二醛(MDA)和超氧化物歧化酶(SOD)含量的影响,探索MODS新的防治途径。方法SD大鼠57只,随机分为对照组、MODS模型组、肠功能恢复汤治疗组和氨苄西林治疗组,分别检测各组外周静脉血、门静脉血及回肠内容物的内毒素含量,测定血清MDA、SOD含量和ALT、AST、Cr及BUN含量,同时取肠系膜静脉血、肠系膜淋巴结、肝、肾、肺和脾组织进行细菌培养,观察细菌移位情况,取回肠组织制成石蜡切片,HE染色,光镜下观察。结果模型组、肠功能恢复汤组和氨苄西林组的肝肾功能受到明显的损害,回肠黏膜不同程度发生上皮细胞水肿、坏死、脱落、绒毛损伤断裂、炎细胞浸润;肠功能恢复汤组与模型组相比,外周血、门静脉血及回肠内容物的内毒素含量、血清MDA、ALT、AST、Cr及BUN含量均显著降低(P<0.01),SOD含量显著升高(P<0.01),但氨苄西林组与模型组相比上述指标差异不明显(P>0.01)。在模型组与氨苄西林组内,MDA和SOD的含量呈负相关(P<0.01)。肠功能恢复汤组和氨苄西林组的细菌移位率均比模型组低,二者间无显著性差异(P>0.01)。结论肠功能恢复汤能抑制MODS大鼠的细菌、内毒素移位和氧化-抗氧化失衡。
Objective To investigate the effect of Intestinal Function-recovering Decoction on the bacteria and endotoxin translocation and the content of malondialdehyde （MDA） and superoxide dismutase （SOD） in serum of rats with multiple organ dysfunction syndrome （MODS）. Methods Fifty-seven SD rats were divided into four groups randomly： control group, MODS group, decoction group and ampicillin group. We detected the content of endotoxin in the peripheral vein, portal vein and ileum. Bacterial translocation, change of ileum mucous membrane in pathomorphology and the contents of MDA, SOD, ALT, AST, Cr and BUN in serum in each group were also examined. Results The hepatic and renal function and ileum mucous membranes were significantly damaged in MODS group, decoction group and ampicillin group. Compared with those in MODS group, serum MDA, ALT, AST, Cr, BUN and endotoxin in the peripheral vein, portal vein and ileum in decoction group were significantly lower （P〈0.01）, and serum SOD was all higher （P 〈 0.01）. However, no significant difference was shown between ampicillin group and MODS group （P〉0.01）. It exhibited a negative correlation between serum MDA and SOD in MODS group and ampicillin group （P〈0.01）. Decoction group and ampicillin group had lower bacterial translocation than MODS group, but with no significant difference. Oonclusion The decoction can inhibit bacterial and endotoxin translocation and oxidant/antioxidant imbalance.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2009年第1期119-123,共5页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
陕西省科学技术厅计划资助项目(No.2007K16-07)~~
