摘要
目的:以阿司匹林为配体的聚乙烯亚胺-β-环糊精(PEI-β-CyD)生物复合材料,观察其理化特性和转基因功能。方法:通过N,N’-羰基二咪唑(1,1’-carbonyldiimidazole,CDI)将阿司匹林偶联到PEI-β-CyD载体材料上,制成PEI-β-CyD-ASP复合材料。用1H-NMR、FT-IR、UV和XRD对合成的载体材料进行了化学表征,用凝胶电泳阻滞实验观察PEI-β-CyD-ASP浓缩质粒DNA的能力,用MTT法在A293、B16、Hela细胞上对载体的细胞毒性进行评价,以及体外转染实验。结果:成功合成了以阿司匹林为配体的聚乙烯亚胺-β-环糊精复合材料,在N/P为4∶1时其能有效浓缩质粒DNA;而且其在A293、B16、Hela细胞上的毒性较低,在B16肿瘤细胞上表现出较高的转染效率。结论:以阿司匹林为配体的聚乙烯亚胺-β-环糊精复合材料是低毒、选择性高的非病毒基因载体。
Objective： To develop a novel non-viral gene delivery vector based on PEI-β-CyD as backbone modified with aspirin, and to identify its physicochemical characters. Methods： 1,1 -carbonyldiimidazole （CDI） was used to bind aspirin onto PEI-β-CyD to form PEI-β-CyD-ASP. 1H-NMR, FT-IR, UV and XRD were used to confirm the polymer structure. The ability of condensation was demonstrated by gel retardation assay. MTT assay was used to test the cell viability in B16,Hela and A293 cell lines. Transfection efficiency of the polymer was tested in B16 ceils. Results： The structure of PEI-β-CyD-ASP was confirmed by 1H-NMR, FT-IR, UV and XRD,which efficiently condensed plasmid DNA at the N/P ratio of 4. The copolymer showed low cytotoxicity and high transfection efficiency in B16 cells. Conclusion： The synthesized aspirin -PEI-β-CyD might be a potential gene delivery vector.
出处
《浙江大学学报（医学版）》
CAS
CSCD
北大核心
2009年第1期46-52,共7页
Journal of Zhejiang University(Medical Sciences)
基金
国家高技术研究发展计划(863)项目(2007AA03Z355)
国家重点基础研究规划(973)项目(2004CB518802)
国家自然科学基金项目(30571068)
