摘要
目的:观察阿司匹林和塞来昔布及二者分别联合阿那曲唑对乳腺癌细胞MCF-7增殖的影响,探讨并比较阿司匹林和塞来昔布的抗肿瘤作用。方法:MTT法检测不同浓度的阿司匹林(2.5、5.0和10.0mmol.L-1)和塞来昔布(30、60和120μmol.L-1)分别作用人乳腺癌细胞株MCF-7不同时间(24、48和72h)的细胞增殖抑制率,以不含药物的培养液作为阴性对照组,以阿霉素作为阳性对照组。MTT法检测不同浓度阿那曲唑(0.5和1.0mmol.L-)及其分别联合不同浓度阿司匹林(2.5、5.0和10.0mmol.L-1)和塞来昔布(30、60和120μmol.L-1)作用于MCF-7细胞48h的细胞增殖抑制率,以不含药物组为阴性对照组。结果:①阿司匹林各剂量组细胞增殖抑制率随药物浓度的增加、作用时间的延长而提高(P<0.01)。②塞来昔布各剂量组的细胞增殖抑制率随药物浓度的增加、作用时间的延长而升高(P<0.01)。③10mmol.L-1阿司匹林和120μmol.L-1塞来昔布单独作用72h抑制率分别达68.88%和87.00%;阳性对照组阿霉素2.0μmol.L-1作用72h抑制率达86.30%。④0.5μmol.L-1阿那曲唑与阿司匹林联合各剂量组的抑制率均明显高于0.5μmol.L-1阿那曲唑单药组(P<0.05),0.5μmol.L-1阿那曲唑联合10.0mmol.L-1阿司匹林对MCF-7的抑制率明显高于0.5μmol.L-1阿那曲唑联合2.5、5.0mmol.L-1阿司匹林(P<0.05)。0.5μmol.L-1阿那曲唑分别与60、120μmol.L-1塞来昔布联合组的抑制率明显高于0.5μmol.L-1阿那曲唑单药组及0.5μmol.L-1阿那曲唑与30μmol.L-1塞来昔布联合组的抑制率(P<0.05),0.5μmol.L-1阿那曲唑联合120μmol.L-1塞来昔布对MCF-7的抑制率明显高于0.5μmol.L-1阿那曲唑联合60μmol.L-1塞来昔布(P<0.05)。1.0μmol.L-1阿那曲唑分别联合10.0mol.L-1阿司匹林、60和120μmol.L-1塞来昔布各组的抑制率明显高于1.0μmol.L-1阿那曲唑单药组(P<0.05),1.0μmol.L-1阿那曲唑联合120μmol.L-1塞来昔布对MCF-7的抑制率明显高于1.0μmol.L-1阿那曲唑联合60μm
Objective To discuss and compare the anti-tumor effects of aspirin and celecoxib on breast cancer cell MCF-7 through investigating the effects of aspirin , celecoxib, and combined with anastrozole respectively on the growth of human breast cancer cell MCF-7. Methods The human breast cancer cell MCF-7 were treated with 2.5 , 5.0 , and 10.0 mmol·L^-1 aspirin and 30 , 60 , and 120 μmol ·L^-1 celecoxib for 24, 48, and 72 h respectively, the MCF-7 without treatment with drug was used as negative control group, the MCF-7 treated by ADM was used as positive control group, the inhibitory effect was detected by MTT assay. Besides, the MCF-7 cells were treated by anastrozole （0.5 and 1.0 μmol ·L^-1）, anastrozole （0.5 and 1.0 μmol·L^-1 ） combined with aspirin （2.5 , 5.0 , and 10.0 mmol·L^-1） or celecoxib （30, 60, and 120 μmol ·L^-1） for 48 h, respectively, the inhibitory rate was detected by MTT assay. Results ① The inhibitory rate of the MCF-7 cell line treated with aspirin was reduced compared with controls, which was in time-dependent and dose-dependent manner （P〈0. 01）. ②The inhibitory rate of the MCF-7 cell line treated with celecoxib was reduced compared with controls, which was in time-dependent and dose-dependent manner （P〈0. 01）.③The inhibitory rates of 10 mmol·L^-1 aspirin and 120 μmol·L^-1 celecoxib on MCF-7 were 68. 88% and 87.00% when treated for 72 h, the inhibitory rate of 2. 0 μmol·L^-1 ADM on MCF-7 was 86.30% when treated for 72 h. ④The inhibitory rate of 0.5μmol·L^-1 anastrozole combined with aspirin on MCF-7 was increased compared with that of anastrozole single （P〈0. 05）. The inhibitory rate of 0. 5 μmol·L^-1 anastrozole combined with 10.0 mmol·L^-1 aspirin on MCF-7 was increased compared with that of 0. 5 μmol·L^-1 anastrozole combined with 2.5 mmol·L^-1 or 5.0 mmol·L^-1 aspirin （P〈 0.05）. The inhibitory rates of 0.5 μmol·L^-1 anastrozole combined with 60 μmol·L^-1 or 120 μmol·L^-1 eeleeoxib on MCF-7 were increased compared wi
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2007年第5期901-904,共4页
Journal of Jilin University：Medicine Edition
基金
吴阶平医学基金资助课题(320
2730
0501)
