摘要
目的:建立人结肠癌细胞株裸鼠肝转移模型,研究老鹳草提取物对裸鼠肝转移模型的影响。方法:采用BALB/C裸鼠,Ls174 t人大肠癌细胞株,脾脏切除脾脏种植法建立裸鼠肝转移模型。将36只裸鼠随机分成模型对照组、老鹳草提取物组和羟基喜树碱阳性对照组,给予老鹳草提取物喂养,观察一般情况及肿瘤生长情况;采用巢式逆转录聚合酶链反应(RT-PCR)检测裸鼠循环血中pS2表达;标本作病理组织学检查。结果:①脾脏切除脾脏种植法建立裸鼠结肠癌肝转移模型的成功率为100%,病理组织学证实肝转移癌细胞呈人结肠癌低分化腺癌特征;②老鹳草提取物组裸鼠肿瘤数目及质量明显低于羟基喜树碱组(P<0.05);③老鹳草提取物组裸鼠血中pS2表达为7.598±0.046,明显高于羟基喜树碱组3.794±0.020(P<0.05)。结论:老鹳草提取物可显著降低肝转移裸鼠血中pS2表达,抑制裸鼠结肠癌肝转移的发展,且效果优于羟基喜树碱,可作为大肠癌术后预防复发和肝转移的治疗手段之一。
Objective： To establish nude mice models with the liver metastases of colonic adenocarcinoma and study the effects of Geranium sibirum extracts on them. Methods： Nude mice liver metastases model of colonic cancer was established with human colonic cancer cells line （ Ls 174t）inoculated into mice spleen. 36 nude mice were randomly divided into 3 groups, containing control group, Geranium sibirum extracts group and hydroxycamptothecine group. The weight and size of the mice and growth of the carcinoma were recorded. All specimens were examined histologically, pS2 in blood in nude mice with liver metastasis of colonic carcinoma was detected with nested RT-PCR. Results： The incidence of liver metastasis was 100% in this intrasplenic injection model. The pathological results showed that tumour cells of liver metastases were poorly differentiated human colonic adenocareinoma. In Geranium sibirum extracts group, the tumor number and the weight of liver metastases were significantly lower than those in hydroxycamptothecine group （P 〈 0. 05 ）. Using semiquantitative examination, in Geranium sibirum extracts group, the relative value of pS2 expression in blood was significantly higher than that in hydroxycamptothecine group（ P 〈0. 05 ）. Conclusion： Geranium sibirum extracts can effectively inhibit the occurrence of liver metastases carcinoma and decrease the positive expression of pS2, it also has better effect than hydroxycamptothecine so that Geranium sibirum extracts may become the potential therapeutic strategy for liver metatstases of colonic cancer.
出处
《中药材》
CAS
CSCD
北大核心
2009年第1期97-99,共3页
Journal of Chinese Medicinal Materials
关键词
老鹳草
结肠癌
肝转移
裸鼠
PS2
RT-PCR
Geranium sibirum
Colonic carcinoma
Liver metastases
Nude mice
pS2
RT-PCR
