摘要
目的探讨儿童单纯性肥胖与纤维蛋白原(Fg)及其Bβ-148C/T基因多态性的关系,为儿童单纯性肥胖的防治提供理论依据。方法选取2004年6月至2007年9月华北煤炭医学院附属医院单纯性肥胖儿童及正常对照组儿童各106例,抽取空腹静脉血5mL,测定Fg水平和分子功能;采用聚合酶链反应及限制性酶切方法对FgBβ-148C/T位点的基因型进行测定。结果单纯性肥胖儿童血浆Fg水平及分子功能明显高于健康对照组(P<0.05),儿童单纯性肥胖T等位基因频率明显高于健康对照组(P<0.05),而且儿童单纯性肥胖组CT与TT基因型个体的血浆Fg水平及Fg单体聚合速度(FMPV)明显高于CC基因型组(P<0.05)。结论儿童肥胖能引起血浆Fg水平增高,分子功能增强,FgBβ-148C/T基因多态性通过影响Fg水平及单体聚合速率可能为儿童单纯性肥胖的一个累效基因。
Objective To study the change of plasma fibrinogen level and the molecular activity of fibrinogen in children with simple obesity. To explor the relationship between the simple obesity and fibrinogen Bβ-148C/T gene polymorphism in children and to provide basis for children＇s simple obesity prevention. Methods A total of 106 children with simple obesity and 106 normal weight children were selected in the Affiliated Hospital of North China Coal Medical College from June 2004 to September 2007.5 mL fast blood sample was taken from each patient. Plasma fibrinogen level and molecular reactivity were measured with Assist Plasm Fibrinogen Activity Assay System. Polymerize chain reaction and restriction enzyme digestion were used to detect the fibrinogen Bβ-148C/T gene polymorphism genes genotype. Results The plasma fibrinogen level and the molecular activity of the children with simple obesity were significantly higher than tbat of the health control group （ P 〈 0.05 ）. The allele frequency in the children with simple obesity was higher than that of the health control group （P 〈 0.05）. The plasma fibfinogen level and the fibrin monomer polymerize velocity of theBβ-148 TT＋CT was higher than that of the CC type in the children with simple obesity. Conclusion The simple obesity in chihlren is associated with the high level and the strengthen molecular activity of fibrinogen. The fibrinogen Bβ-148C/T gene polymorphism may be an accumulative efficiency gene of children with simple obesity by influencing the level and the fibrinogen metamer polymerization velocity.
出处
《中国实用儿科杂志》
CSCD
北大核心
2009年第2期116-118,共3页
Chinese Journal of Practical Pediatrics
基金
河北省科技攻关项目(062761924)
