摘要
目的探讨钠氢交换蛋白1(NHE1)mRNA在原发性肝细胞肝癌组织及癌旁组织的表达及意义。方法采用RT-PCR检测诊断为HCC患者34例的肝癌及癌旁组织中NHE1 mRNA的表达情况。结果所有肝癌组织和癌旁组织NHE1均有表达,相对表达量分别为2.892±0.882和0.802±0.206,两者存在显著性差异(P<0.001)。作为对照的21例肝组织NHE1的相对表达量为0.872±0.186。肝癌组织与对照肝组织比较存在显著性差异(P<0.001);癌旁组织与对照肝组织比较差异无统计学意义(P>0.10)。将从同一患者取材的肝癌组织和癌旁组织检测到的NHE1 mRNA的表达量进行配对t检验,两者间存在显著性差异(P<0.001)。结论NHE1 mRNA在肝癌组织的表达增高,可能在肝癌的发生中起一定的作用。
Objective To study the expression of Na＋/H ＋ exchanger 1 （NHE1） mRNA in primary hepatocellular carcinoma （HCC） tissues, and to discuss its value in the development of HCC. Methods RT-PCR was used to detect the expression of NHE1 mRNA in the tissues of 34 HCC and its paraneoplastie tissues and 21 non-HCC liver tissues from the other patients. Results NHE1 mRNA was expressed widely. The relative intensities of its expression in HCC, paraneoplastic tissues, non-HCC liver tissues were respectively 2. 892 ±0. 882, 0. 802±0. 206 and 0. 872 ±0. 186. Conclusion NHE1 mRNA may play an important role for the development of HCC.
出处
《胃肠病学和肝病学杂志》
CAS
2009年第2期121-122,共2页
Chinese Journal of Gastroenterology and Hepatology
基金
国家自然科学基金(304004425)
陕西省科技研究发展计划项目(2008k09-05)
关键词
肝细胞癌
钠氢交换蛋白1
Hepatocellular carcinoma
Na ＋/H ＋ exchanger 1 （ NHE1）
