摘要
目的观察有机磷农药中毒（AOPP）小鼠肝脏、脾脏中细胞因子信号转导抑制因子3（SOCS-3）和肿瘤坏死因子α（TNF-α）mRNA表达的变化，探讨SOCS-3，TNF-α在AOPP致多器官功能衰竭（MODS）中的发病机制，从而为MODS的防治提供新的策略。方法36只健康雄性BALB／c小鼠制成中毒模型，随机分成敌敌畏中毒组（n=12），生理盐水对照组（n=12），正常对照组（n=12），分别于染毒后2h，6h，12h，24h取小鼠的肝脏、脾脏，以逆转录聚合酶链反应（RT-PCR）测定SOCS-3和TNF-α表达水平，各组间比较采用方差分析进行。结果中毒后2，6，12和24h其肝、脾中SOCS-3基因的mRNA表达量均明显高于对照组（P〈0．05），其中肝脏中24h达到最高峰，脾脏中于12h达最高峰，之后到24h有下降（P〈0．05）。肝、脾中TNF-α基因的mRNA表达量均明显高于对照组（P〈0．05），并于12h达最高峰，之后到24h有下降（P〈0．05）。AOPP中毒后肝脏和脾脏组织的RNA条带为5S，15S和30S，参比基因β-actin在鼠肝脏和脾脏中的mRNA表达，中毒后2h，6h，12h，24h其肝、脾中SOCS-3基因的mRNA表达，其中肝脏中24h达到最高峰，脾脏中于12h达最高峰，之后到24h有下降（P〈0．05）。肝、脾中TNF-α基因的mRNA表达于12h达最高峰，之后到24h有下降（P〈0．05）。统计分析显示，肝脏中SOCS-3和TNF-α mRNA存在明显的正相关性（y=0．089＋0．758x，r=0．939，F=252．168，P〈0．01），脾脏中SOCS3和TNF-α mRNA存在明显的正相关性（y=0．057＋0．361z，r=0．953，F=336．122，P〈0．01）。结论不同时间点，AOPP中毒小鼠肝脏，脾脏中SOCS-3，TNF-α mRNA表达趋势一致。小鼠肝脾SOCS-3和TNF-α水平均升高。
Objective To investigate the dynamic expression of SOCS-3, TNF-α in live and spleen of mice induced by AOPP, and to discuss the mechanism of MODS induced by AOPP, so that to give some intervention method over MODS in the future. Method Thirty-six adult BALB/c mice were divided into 3 groups randomly： AOPP group （ n = 12）. Water normal group （ n = 12） and normal group （ n = 12）. After post poison 2 hours, 6 hours, 12 hours and 24 hours, the liver and the spleen tissue were taken out. The expression of SOCS-3 and TNF-α were detected by RT-PCR. And data were analyzed with ANOVA. Results After AOPP of 2,6,12,24 hours,the mRNA expression of SOCS-3 increased obviously in the liver and spleen compared with the normal group （ P 〈 0.05）, it reached peak at the 24th hour in liver,and reached peak at the 12th hour in spleen,then descended at 24th hour （ P 〈 0.05）. The mRNA expression of TNF-α increased obviously in the liver and spleen than normal group （P 〈 0.05）, and reached peak at the 12th hour,then descended at 24th hour （P 〈 0.05）, the electrophoresis image of RNA was 5 s 15 s and 30 s,RT-PCR amplification of β-actin showed,the expression of SOCS-3, reached peak at the 24th hour in liver and it reached peak at the 12th hour in spleen,then descended at 24th hour （ P 〈 0.05）, TNF-α reached peak at 12th hour in the liver and spleen, then descended at 24th hour. By statistic analysis, positive correlation was indicated among SOCS-3 and TNF-α mRNA expression in liver （y = 0.089 ＋ 0. 758x, r = 0.939, F = 252. 168, P 〈 0.01） positive correlation was indicated among sots-3 and TNF-α mRNA expression in spleen （ y = 0. 057 ＋ 0. 361x, r = 0. 953, F = 336. 122, P 〈 0.01 ）. Conclusions At different point of time after Aopp, the mRNA expression of sots-3 and TNF-α showe the same trend in liver and spleen, the levels of SOCS-3, TNF-α all increase significently.
出处
《中华急诊医学杂志》
CAS
CSCD
北大核心
2009年第2期175-179,共5页
Chinese Journal of Emergency Medicine
基金
海南省自然科学基金（30718）
