摘要
目的:探讨银杏叶提取物对高糖环境下人脐静脉内皮细胞(ECV304)的保护作用及机制。方法:体外培养的ECV304细胞分为高糖组、银杏叶保护组、甘露醇高渗对照组(甘露醇组)和正常细胞组,在35 mmol.L-1高糖条件下培养ECV304细胞24 h后,分别收集不同处理组的细胞,检测细胞产生羟自由基(.OH)、超氧阴离子(O2-)、丙二醛(MDA)水平,同时测定细胞超氧化物歧化酶(SOD)活性;采用不同处理组的细胞制作扫描电镜图片,观察细胞及线粒体形态的改变。结果:高糖组细胞产生.OH、O2-和MDA水平高于银杏叶保护组(P<0.05),SOD活性低于银杏叶保护组(P<0.05)。甘露醇组细胞产生.OH、O2-、MDA水平显著低于高糖组(P<0.05)。电镜下可见银杏叶保护组线粒体形态完好,线粒体内嵴清晰可见;高糖组线粒体肿胀,线粒体内嵴消失,坏死细胞增多。结论:银杏叶提取物对高糖导致的ECV304细胞损伤具有保护作用。
Objective To explore the protective effects of Ginkgo biloba extract （GBE） on ECV304 cells exposed to high-level glucose and its mechanisms . Methods ECV304 cells were divided into four groups： high level glucose group, GBE protective group, mannitol high-osmosis group and normal group. In vitro, ECV 304 cells were exposed to 35 mmol · L^-1 glucose for 24 h. The cells from different groups were collected, the production of super anion （O2^-）, hydroxyl radical （ · OH）, level of malonaldehyde （MDA）, and activity of superoxide dismutase （SOD） were determined. The structures of mitochondria of cells in different groups were also observed under electronic microscope. Results The level of MDA and the production of O2^- , · OH in ECV304 cells in high-level glucose group were significantly higher than those in GBE group （P〈0.05）, the SOD activity was significantly lower than that in GBE group （P〈0.05）. The level of MDA and production of O2^- , ·OH in mannitol group were lower than those in high-level glucose group （P〈0. 05）. Under electronic microscope, the mitochondrial structure of ECV 304 cells in GBE group was normal and cristea were clear and in perfect shape, but the mitochondrial structure of ECV 304 cells in high-level glucose group was swollen and cristea were disappeared. Conclusion GBE plays a protective role in ECV 304 cells exposed to high-level glucose.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2009年第1期131-134,共4页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科研基金资助课题(20070919-1)
长春市科学技术局科研基金资助课题(2007GH2033)
关键词
银杏叶提取物
高糖
线粒体
自由基
电镜观察
ginkgo biloba extract
high glucose
mitochondria
free radicals
electronic microscope observation
