摘要
目的研究电刺激小脑顶核(FNS)对阿尔茨海默病(AD)大鼠学习记忆能力及脑内海马神经元c-Rel和Bcl-xl表达的影响。方法40只SD大鼠随机分成4组:Aβ1-40微量注射组(AD组)、假手术组(SC组)、Aβ1-40微量注射+电刺激小脑顶核组(FNS组)和Aβ1-40微量注射+电刺激齿状核组(DNS组),采用Morris水迷宫检测电刺激小脑顶核对AD大鼠认知功能的影响;通过HE染色、电镜观察电刺激小脑顶核对AD大鼠的海马神经元形态的影响;采用免疫组化法观察电刺激小脑顶核对AD大鼠海马神经元c-Rel和Bcl-xl的表达的影响。结果与SC组比较,AD组、FNS组和DNS组每天隐匿平台逃避潜伏期明显延长(P<0.05)、海马CA1区的Bcl-xl和c-Rel阳性细胞OD值差异显著(均P<0.05);FNS组与AD组比较,大鼠每天逃避潜伏期明显缩短(P<0.05),FNS组海马CA1区Bcl-xl和c-Rel阳性细胞OD值为(0.46±0.04、0.65±0.09),较AD组阳性细胞OD值明显增高(P<0.05),但齿状核刺激(DNS)组与AD组比较大鼠每天逃避潜伏期和海马CA1区Bcl-xl和c-Rel阳性细胞OD值无显著差异(P>0.05)。结论电刺激小脑顶核能通过增强C-Rel及Bcl-xl的表达,抑制海马神经元的凋亡,改善AD大鼠学习记忆能力。
Objective To study the effects of fastigial nucleus electrostimulation （FNS） on the abilities of learning and memory and the expressions of C-Rel and Bcl-xl protein of hippocampal neurons in Alzheimer＇ s disease （ AD ） rats induced by β-amyloid （ Aβ1-40 ）. Methods Forty SD rats were randomly divided into： Aβ1-40 micro-injection （AD）, sham （SC）, Aβ1-40 micro-injection and FNS （FINS） and Aβ1-40 micro-injection and corpus dentatum electrostimulation （DNS） groups. The effects of FNS on the abilities of learning and memory of the AD rats were measured by Morris water maze test. The appearance of AD rats hippocampal neurons were observed by HE staining and e- lectron microscope. The expressions of c-Rel and Bcl-xl protein of hippocampal neurons were detected by immunohistochemistry method. Results The escape latency of the place navigation in AD, FNS and DNS groups were singnificantly longer than those in SC group（ P 〈 0.05 ）, the OD value of positive cells of c-Rel and Bcl-xl in hippocampus CA1 area of AD, FNS and DNS groups were obviously different from those of SC group. The escape latency was shorter in FNS group than that in AD group（P 〈0. 05）, the OD value of c-Rel and Be]-xl in hippoeampus CA1 area of FNS group （0. 65 ±0. 09, 0. 46 ±0. 04 ） were increased than those of AD group （ P 〈 0. 05 ）. The OD value of c-Rel and Bcl-xl in hippocampus CA1 area and the escape latency were not different between DNS group and AD group（ P 〉0. 05 ）. Conclusions FNS could improve the abilities of learning and memory of AD rats by increasing the expressions of c-Rel and Bcl-xl and inhibiting hippocampal neurons apoptosis.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2009年第3期257-260,共4页
Chinese Journal of Gerontology
基金
重庆市科委自然科学基金(CSTC2007BB5290)
