摘要
目的观察应力对纯钛表面成骨细胞核结合因子α1(Cbfα1)形成及表达的影响,揭示应力对支抗种植体周骨反应的调控机制。方法用大小为2.205N的间断离心力刺激接种于纯钛表面的成骨细胞,刺激频率为每培养4h加力15min,分别于4、8、12h后收集细胞,采用免疫荧光法检测Cbfα1的形成,提取总RNA并应用逆转录聚合酶链反应检测Cbfα1mRNA的表达。结果纯钛表面加力组和不加力组MG-63成骨细胞的细胞核内均有Cbfα1荧光染色,且加力组Cbfα1蛋白荧光强度较不加力组多,差异有统计学意义(P<0.05);纯钛表面加力组和不加力组MG-63成骨细胞Cbfα1mRNA表达均为阳性,且加力组Cbfα1mRNA表达较不加力组多,差异有统计学意义(P<0.05)。结论成骨细胞受力后Cbfα1基因形成和表达增强,提示间歇性的牵引应力可以促进纯钛表面成骨细胞的活性。
Objective To evaluate the effects of mechanical stress on the formation and expression of core bind- ing factor α1 （Cbfα1） in MG-63 cells cultured on titanium in vitro. Methods MG-63 cells cultured on the titanium were subjected to a centrifugal force（2.205 N） 15 rain per 4 hours and collected after 4, 8 and 12 hours. The formation and expression of Cbfα1 were examined by immunofluorescence staining and reverse transcription-polymerase chain reaction （RT-PCR）. Results Both the cells with or without centrifugal force created the fluorescence in the nucleus and the immunofluorescence intensity of Cbfα1 in MG-63 cells with centrifugal force were higher than those without centrifugal force （P〈0.05）. Meanwhile, both the cells with or without centrifugal force expressed the mRNA of Cbfα1 and the relative mRNA level of Cbfal in MG-63 cells with centrifugal force were higher than those without centrifugal force, and the differences were great significant（P〈0.05）. Conclusion Mechanical stress are beneficial to the formation and expression of Cbfα1.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2008年第6期588-590,598,共4页
West China Journal of Stomatology
关键词
应力
钛
成骨细胞
核结合因子α1
mechanical stress
titanium
osteoblast
core binding factor α1
