摘要
目的 :研究紫外线辐射对小鼠血清肿瘤坏死因子 α(TNFα)水平和脾细胞 TNFα m RNA表达的影响 ,从而探讨紫外线辐射所致免疫抑制的作用机制。方法 :用酶联免疫吸附试验检测小鼠血清中 TNFα含量。一步法提取脾细胞 RNA,逆转录聚合酶链反应检测其 TNFα m RNA表达。结果 :一定剂量的紫外线辐射不能使小鼠血清中出现可检测的 TNFα(P>0 .0 5 )。但可使诱生的 TNFα水平下降 (P<0 .0 1) ,辐射剂量与 TNFα的浓度呈直线负相关 (P<0 .0 1)。脾细胞 TNFα m RNA表达被抑制 (P<0 .0 0 1)。结论 :紫外线辐射可能是通过抑制具有分泌 TNFα功能的淋巴细胞和 (或 )单核巨噬细胞的 TNFα m RNA转录 ,进而抑制 TNFα的分泌 。
Objective:To investigate the mechanisms of immunosuppression induced by ultraviolet radiation, and observed the influence of ultraviolet radiation on serum TNFα level and spleen cells TNFα mRNA expression in mice. Methods:Serum TNFα was detected by Enzyme Linked Immuno Sorbent Assay (ELISA) method. A step extraction method was used to get RNA of spleen cells. mRNA expression of TNFα was assayed by RT PCR test. Results: Ultraviolet radiation failed to induce a detectable rise in circulating TNFα in mice( P>0.05 ). TNFα secretion was significantly inhibited by ultraviolet radiation ( P<0.01) . The dosage of ultraviolet radiation was negatively correlated with the serum TNFα level ( P<0.01) , and the mRNA expression of TNFα in spleen cells was suppressed by ultraviolet radiation ( P<0.001) . Conclusion:The results suggest that ultraviolet radiation may suppress TNFα mRNA transcription in macrophage or activate lymphocytes further to diminish its TNFα secretion and finally influence some immune responses.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第3期203-205,210,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金资助项目 ( 3 9670 676)
