摘要
目的:探讨转录因子Oct4及组织蛋白酶B(CathepsinB,CB)在人脑胶质瘤中的表达和预后的关系,并探讨Oct4的表达在脑胶质瘤发生发展中的意义及与肿瘤干细胞的关系。方法:采用免疫组织化学S-P法检测51例人脑胶质瘤组织和8例正常人脑组织中Oct4,CB的表达。结果:Oct4及CB的表达在正常脑组织与胶质瘤之间以及胶质瘤的高低级别组间存在显著差异(P<0.05),并且在生存时间>2年组与生存时间≤2年组间差异显著(P<0.05),且两者的表达呈显著正相关(P<0.01)。在单因素分析中,Oct4的表达与患者预后不良有关。结论:Oct4的高表达在胶质瘤的恶变进展,增殖和侵袭过程中起着重要作用,可作为胶质瘤恶性表型的有用指标;Oct4阳性表达与患者预后不良有关。Oct4可能与CB的活化密切相关,直接或间接促进脑胶质瘤的侵袭。
Objective ： To explore the expressions of Oct4 and CathepsinB in human cerebral gliomas and their relationship to prognosis of glioma patients, and demonstrate the significance of Oct4 in the formation of glioma and relationship with cancer stem cells. Methods： The expressions of Oct4 and CathepsinB in 51 cases of human glioma tissues and 8 cases of normal brain tissues were determined by immunohistoehemical S - P technique. Results： The positive expression rates of Oct4 and CathepsinB in human gliomas of grades Ⅰ - Ⅱ were significantly higher than those in normal brain tissue （P 〈 0.05 ） , and significantly lower than those in human gliomas of grades Ⅲ - Ⅳ （P 〈 0. 05 ）. The expression of Oct4 was a considerable factor in survival univariate analysis. Conclusion： Oct4 is over expressed in malignant glioma, especially in glioblastoma tissues, and may involve in the progression and invasion of glioma. It can be regarded a useful marker for judging the malignant phenotype of glioma. Moreover, expression of Oct4 can provide useful prognostic information. It may be implicated in the activation of CathepsinB and facilitate invasion of human glioma directly or indirectly.
出处
《现代肿瘤医学》
CAS
2009年第2期239-242,共4页
Journal of Modern Oncology
