摘要
目的研究骨髓间充质干细胞经冠状动脉内移植的安全性及可行性。方法左侧开胸结扎SD大鼠左冠状动脉,2周后取心脏建立Langerndorff模型。将CM-DiI标记的骨髓间充质干细胞悬液注入主动脉根部,同时收集右心房回流的液体,离心后通过流式细胞仪计数细胞数量。观察是否有细胞经冠状静脉回流及数量比例。并在不同时间测LVSP、LVDP、±dp/dt、心率,评价其安全性。在体实验中取心梗后2周的大鼠,经左心室-主动脉途径将细胞悬液注射到临时阻断的主动脉根部,分别在移植后1及24 h和1及4周取材观察细胞在心脏内的位置及细胞的迁移情况。结果离体实验发现经冠状动脉内注射骨髓间充质干细胞90%以上的细胞在心肌组织内存留,移植后LVSP、LVDP、±dp/dt、心率没有明显变化。在体内实验中发现细胞移植后早期大部分细胞分布在心外膜下心肌组织内,在心内膜下组织内较少细胞分布,而且大部分细胞在正常心肌组织内,只有少量在心梗区域。而在细胞移植后1-4周存活的细胞多在心肌梗死及交界区组织内,在正常组织内很少有移植细胞存在。结论经冠状动脉途径进行细胞移植是安全可行的。
Objective To investigate the retainage rate, distribution, and emigration of the bone marrow mesenchyreal stem cell （BMMSC） after transeoronary infusion and to further evaluate the feasibility of injecting BMMSCs into coronary artery. Methods BMMSCs were isolated, purified, expanded, and labelled with CM-Dil. The infarcted SD rat hearts were removed and perfused with Langendorff apparatus. The cells were injected into the aortic root and the fluid returning from the coronary system was collected and the labelled cells in the coronary effluent were quantitated with flowcytometry. At the same time, left ventricle function was recorded to evaluate the safety of this approach. In vivo study, the cells were then injected into clamped ascending aorta through a catheter inserted through the left ventricle into the aortic root. The hearts were harvested at different time points after cell transplantation to obtain the direct evidence of distribution and emigration of the implanted cells. Results Only 3% -5% of transplanted cells returned into the fight ventricle and more than 90% cells retained in the heart after being injected into aortic root of Langendorff model of infarcted hearts of SD rats. Left ventricle function did not deteriorate after cell transplantation. The labelled cells were entrapped within the coronary capillary immediately after cell infusion, mainly in the normal area. After 24 hours some cells migrated through the capillary wall into interstitium of the heart. One week later we found that most survival cells located at the infarcted area and the border zone. Conclusion The majority of BMMSCs delivered by transcoronary infusion retained in the heart. BMMSCs can penetrate the vessel wall and home back to the interstitial compartment and the injured area in a few hours.
出处
《基础医学与临床》
CSCD
北大核心
2009年第1期69-73,共5页
Basic and Clinical Medicine
关键词
心肌梗死
细胞移植
骨髓间充质干细胞
myocardial infarction
cell transplantation
bone marrow mesenchymal stem cell
